Aggregated DNA in ethanol solution
1995; Wiley; Volume: 375; Issue: 3 Linguagem: Inglês
10.1016/0014-5793(95)01206-t
ISSN1873-3468
Autores Tópico(s)Bacteriophages and microbial interactions
ResumoA recently developed mechanochemical method has provided a new, efficient tool for studies on the thermal stability and structure of aggregated DNA in ethanol‐water solutions. At low ethanol concentrations DNA is fully soluble and is in the B form. However, with increasing ethanol concentration the melting temperature of DNA, T m , decreases. At a critical ethanol concentration dependent on the nature and concentration of the counterion, aggregation of the DNA molecules sets in. This is reflected in a marked increase in T m indicating that the aggregated DNA molecules are thermally more stable than the dissolved ones. However, they are still in the B form. In general, T m of aggregated DNA also decreases with further increasing ethanol concentration and is dependent on the nature of the counterion, but T m is not affected by the concentration of the counterion (excess salt) in the ethanol‐water solution. When the ethanol concentration reaches the range of 70–80% (v/v), the B‐to‐A conformational transition occurs in the case of Na‐, K‐ and CsDNA. Above this transition point the A form is more stable than the B form due to the reduced water activity and to increased interhelical interactions. At very high ethanol concentrations, above 85% and dependent on the nature of the counterion, a drastic change in the thermal behaviour is observed. Apparently such a strong interhelical interaction is induced in the aggregated DNA that the DNA is stabilized and cannot adopt a random coil state even at very high temperatures. This stability of DNA in the P form is fully reversed if the ethanol concentration is lowered and the activity of water, thereby, is restored.
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