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Microbial Diversity in Acidic Anaerobic Sediments at the Geothermal Caviahue-Copahue System, Argentina

2013; Trans Tech Publications; Volume: 825; Linguagem: Inglês

10.4028/www.scientific.net/amr.825.7

1662-8985

Graciana Willis-Poratti, Sabrina Hedrich, Iván Ñancucheo, D. Barrie Johnson, Edgardo Donati,

Minerals Flotation and Separation Techniques

In this work we have examined the bacterial diversity from the hot spring sediment Agua del Limón (AL1) present at the geothermal Caviahue-Copahue system using a combination of molecular and cultivation techniques, with particular emphasis on indigenous anaerobic prokaryotes. Microorganisms involved in the iron ( Acidithiobacillus ferrooxidans and Leptospirillum spp.) and sulphur ( Acidithiobacillus spp. , Thermotogales -like bacteria, Thiomonas sp., and Desulfurella sp . ) cycles were identified in the clone library. Although no obvious sulfate-reducing bacteria were detected by culture-independent techniques, several isolates related to the mesophilic, spore-forming sulfate-reducer "Desulfobacillus acidavidus" strain CL4 were isolated at 30°C and 40°C. The 16S rRNA gene of another isolate showed 94% similarity to Desulfotomaculum thermobenzoicum . Sulfate-reducing enrichment cultures of the Copahue samples were also dominated by "Dsb. acidavidus" CL4.