Urotensin II-immunoreactivity in the brainstem and spinal cord of the rat
2001; Elsevier BV; Volume: 305; Issue: 1 Linguagem: Inglês
10.1016/s0304-3940(01)01804-3
ISSN1872-7972
AutoresS.L. Dun, G. Cristina Brailoiu, Jun Yang, J.K. Chang, Nae J. Dun,
Tópico(s)Biochemical effects in animals
ResumoThe distribution of urotensin-II-immunoreactivity (irU-II) was studied in the rat brainstem and spinal cord with the use of an antiserum against the human urotensin II (U-II) peptide. A population of ventral horn neurons in the spinal cord, hypoglossal nucleus, dorsal motor nucleus of the vagus, facial motor nucleus, nucleus ambiguus, abducens nucleus and trigeminal motor nucleus exhibited irU-II of varying intensities. The number of irU-II motor neurons was higher in the lumbar segments as compared to that of cervical, thoracic and sacral segments. Double-labeling the sections with U-II- and choline acetyltransferase (ChAT)-antisera revealed that nearly all irU-II ventral horn and brainstem neurons were ChAT-positive. The result provides the first immunohistochemical evidence of the presence of irU-II in cholinergic motoneurons of the rat spinal cord and brainstem.
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