Two-dimensional analysis of copolymers by size-exclusion chromatography and gradient-elution reversed-phase precipitation chromatography
1984; Elsevier BV; Volume: 317; Linguagem: Inglês
10.1016/s0021-9673(01)91700-5
ISSN1873-3778
AutoresGottfried Glöckner, J. H. M. van den Berg, Nico Meijerink, Theo G. Scholte, R. Koningsveld,
Tópico(s)Microfluidic and Capillary Electrophoresis Applications
ResumoPoly(styrene—co-acrylnitrile) samples were fractionated by size-exclusion chromatography and subsequent high-performance precipitation liquid chromatography (HPPLC) of effluent fractions. HPPLC was performed on a reversed phase column by using a mobile-phase composition gradient with eluting power increasing from 2,2,4-trimethylpentane as a non-solvent to tetrahydrofuran as a solvent. A mixture of two model copolymers of 23 and 30 wt. % acrylonitrile, having molar masses of 825 and 190 kg/mol, respectively, and a commercial SAN copolymer were studied. The correction of retention volumes for the influence of molar mass was determined, and applied in the evaluation of the data for the commercial sample.
Referência(s)