Determination of isoniazid in plasma by liquid chromatography
1988; Elsevier BV; Volume: 425; Linguagem: Inglês
10.1016/0378-4347(88)80053-7
ISSN1872-812X
Autores Tópico(s)Pesticide Residue Analysis and Safety
ResumoIsoniazid, pyridine-4-carboxylic acid hydrazide, is an antituberculosis-agent, which is used to prevent the development of clinical tuberculosis. A validated square-wave adsorptive cathodic stripping voltammetric procedure for the trace determination of the bulk drug at the hanging mercury drop electrode (HMDE) has been developed. Under the optimized conditions, (accumulation potential=−0.9 V, accumulation time=50–300 s, scan increment=8 mV, pulse-amplitude=25 mV, frequency=120 Hz and acetate buffer at pH 5.5) isoniazed generated two irreversible cathodic peaks. The first peak current showed a linear dependence with the drug concentration over the range 5×10−10–2×10−6 M. The mean percentage recoveries, based on the average of five replicate measurements, for 7×10−9 and 5×10−8 M isoniazid were 97.71±2.93 and 99.76±0.77, respectively. The achieved limits of detection (LOD) and quantitation (LOQ) were 1.18×10−10 and 3.93×10−10 M isoniazid, respectively. The procedure was applied to the assay of the drug in tablets (Isocid and T.B. Zide), spiked human serum and urine with mean percentage recoveries of 97.81±1.49, 97.45±2.09, and 97.08±1.06, respectively. The limits of detection of 1.47×10−9 and 2.4×10−8 M, and quantitation of 4.9×10−9 and 8×10−8 M drug in human serum and urine, respectively, were achieved. The mean values of the various pharmackinetic parameters of isoniazid (Cmax, Tmax, t1/2, AUC, and Ke), estimated from analysis of plasma of two volunteers by means of the proposed procedure were similar to literature values.
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