Intracellular Inducible Alkylation System That Exhibits Antisense Effects with Greater Potency and Selectivity than the Natural Oligonucleotide
2006; Wiley; Volume: 45; Issue: 19 Linguagem: Inglês
10.1002/anie.200504441
ISSN1521-3773
AutoresMd. Monsur Ali, Motoi Oishi, Fumi Nagatsugi, Kenya Mori, Yukio Nagasaki, Kazunori Kataoka, Shigeki Sasaki,
Tópico(s)Advanced biosensing and bioanalysis techniques
ResumoOne mismatch is discriminated in a target mRNA sequence by an inducible alkylation system based on sulfide precursors to the nucleoside 2-amino-6-vinylpurine (see scheme). The reactive oligonucleotides were delivered into the cell as poly(ethylene glycol) (PEG) conjugates in polyion-complex (PIC) micelles and showed antisense activity of high selectivity and greater potency than that of the natural antisense oligonucleotide.
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