The Involvement of the μ-Opioid Receptor in Ketamine-Induced Respiratory Depression and Antinociception
2001; Lippincott Williams & Wilkins; Volume: 93; Issue: 6 Linguagem: Inglês
10.1097/00000539-200112000-00031
ISSN1526-7598
AutoresElise Sarton, Luc J. Teppema, C. N. Olievier, Diederik Nieuwenhuijs, Hans W. D. Matthes, Brigitte L. Kieffer, Albert Dahan,
Tópico(s)Neurotransmitter Receptor Influence on Behavior
ResumoN-methyl-d-aspartate receptor antagonism probably accounts for most of ketamine's anesthetic effects; its analgesic properties are mediated partly via N-methyl-d-aspartate and partly via opioid receptors. We assessed the involvement of the μ-opioid receptor in S(+) ketamine-induced respiratory depression and antinociception by performing dose-response curves in exon 2 μ-opioid receptor knockout mice (MOR−/−) and their wild-type littermates (WT). The ventilatory response to increases in inspired CO2 was measured with whole body plethysmography. Two antinociceptive assays were used: the tail-immersion test and the hotplate test. S(+) ketamine (0, 10, 100, and 200 mg/kg intraperitoneally) caused a dose-dependent respiratory depression in both genotypes, with greater depression observed in WT relative to MOR−/− mice. At 200 mg/kg, S(+) ketamine reduced the slope of the hypercapnic ventilatory response by 93% ± 15% and 49% ± 6% in WT and MOR−/− mice, respectively (P < 0.001). In both genotypes, S(+) ketamine produced a dose-dependent increase in latencies in the hotplate test, with latencies in MOR−/− mice smaller compared with those in WT animals (P < 0.05). In contrast to WT mice, MOR−/− mice displayed no ketamine-induced antinociception in the tail-immersion test. These results indicate that at supraspinal sites S(+) ketamine interacts with the μ-opioid system. This interaction contributes significantly to S(+) ketamine-induced respiratory depression and supraspinal antinociception.
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