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A comparison of the ability of β-galactosidase and horseradish peroxidase enzyme-antibody conjugates to detect specific antibodies

1979; Elsevier BV; Volume: 31; Issue: 3-4 Linguagem: Inglês

10.1016/0022-1759(79)90137-6

1872-7905

Michael O’Sullivan, E. Gnemmi, A. D. Simmonds, G. Chieregatti, E Heyderman, James Winfred Bridges, V. Marks,

Cytomegalovirus and herpesvirus research

The ability of two different enzyme-antibody conjugates to detect specific antibodies has been compared. β-Galactosidase was conjugated to antibodies raised against rabbit Fc fragments using m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS). Horseradish peroxidase (HRP) was conjugated to part of the same batch of antibodies using the periodate method. The β-galactosidase and the HRP labels enabled detection of approximately 8 fmoles and 4 fmoles respectively of human growth hormone (HGH) antibodies, when their enzyme activities were measured spectrophotometrically. The detection limit of the β-galactosidase label was increased 4-fold when a fluorimetric detection system was employed.