Retinoblastoma Protein Prevents Staurosporine-Induced Cell Death in a Retinoblastoma-Defective Human Glioma Cell Line
2007; Karger Publishers; Volume: 74; Issue: 1 Linguagem: Inglês
10.1159/000101048
ISSN1423-0291
AutoresFumiyuki Yamasaki, Yoshinori Kajiwara, Seiji Hama, Taro Murakami, Toshikazu Hidaka, Taiichi Saito, Hiroyuki Yoshioka, Kazuhiko Sugiyama, Kazunori Arita, Kaoru Kurisu,
Tópico(s)Ocular Oncology and Treatments
Resumo<i>Objective:</i> To investigate the mechanism of staurosporine-induced glioma cell death and cell cycle arrest using adenovirus-mediated gene transfection, as well as the function of retinoblastoma (Rb) and genetic instability induced by staurosporine. <i>Methods:</i> Cell cycle regulation, cell death and nuclear abnormalities induced by staurosporine were examined using an adenovirus vector expressing <i>Rb, p16</i> or <i>p21 </i>genes in human glioma cell lines. <i>Results:</i> The Rb-defective SF-539 cell line was resistant to staurosporine compared with cell lines expressing intact Rb. SF-539 glioma cells exposed to staurosporine became multinucleated and then died. Multinucleation was prevented in SF-539 cells transfected with the <i>Rb</i> gene, thus decreasing the death rate of these cells. <i>Conclusions:</i> These results imply that enforced Rb expression protects cells from genomic instability induced by staurosporine regardless of its upstream molecular effects.
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