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Model for stathmin/OP18 binding to tubulin

2000; Springer Nature; Volume: 19; Issue: 2 Linguagem: Inglês

10.1093/emboj/19.2.213

1460-2075

Gerlind Wallon, Juri Rappsilber, Matthias Mann, Luis Serrano,

DNA Repair Mechanisms

Article17 January 2000free access Model for stathmin/OP18 binding to tubulin Gerlind Wallon Corresponding Author Gerlind Wallon Structural Biology Program, EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany Search for more papers by this author Juri Rappsilber Juri Rappsilber Protein Interaction Laboratory, University of Southern Denmark, Campusvej 55, 5230 Odense, Denmark Search for more papers by this author Matthias Mann Matthias Mann Protein Interaction Laboratory, University of Southern Denmark, Campusvej 55, 5230 Odense, Denmark Search for more papers by this author Luis Serrano Luis Serrano Structural Biology Program, EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany Search for more papers by this author Gerlind Wallon Corresponding Author Gerlind Wallon Structural Biology Program, EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany Search for more papers by this author Juri Rappsilber Juri Rappsilber Protein Interaction Laboratory, University of Southern Denmark, Campusvej 55, 5230 Odense, Denmark Search for more papers by this author Matthias Mann Matthias Mann Protein Interaction Laboratory, University of Southern Denmark, Campusvej 55, 5230 Odense, Denmark Search for more papers by this author Luis Serrano Luis Serrano Structural Biology Program, EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany Search for more papers by this author Author Information Gerlind Wallon 1, Juri Rappsilber2, Matthias Mann2 and Luis Serrano1 1Structural Biology Program, EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany 2Protein Interaction Laboratory, University of Southern Denmark, Campusvej 55, 5230 Odense, Denmark *Corresponding author. [email protected] The EMBO Journal (2000)19:213-222https://doi.org/10.1093/emboj/19.2.213 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Stathmin/OP18 is a regulatory phosphoprotein that controls microtubule (MT) dynamics. The protein does not have a defined three-dimensional structure, although it contains three distinct regions (an unstructured N-terminus, N: 1–44; a region with high helix propensity, H 1: 44–89; and a region with low helix propensity, H 2: 90–142). The full protein and a combination of H 1 and H 2 inhibits tubulin polymerization, while the combination of H 1 and the N-terminus is less efficient. None of the individual three regions alone are functional in this respect. However, all of them cross-link to α-tubulin, but only full-length stathmin produces high-molecular-weight products. Mass spectrometry analysis of α-tubulin–stathmin/OP18 and its truncation products shows that full-length stathmin/OP18 binds to the region around helix 10 of α-tubulin, a region that is involved in longitudinal interactions in the MT, sequestering the dimer and possibly linking two tubulin heterodimers. In the absence of the N-terminus, stathmin/OP18 binds to only one molecule of α-tubulin, at the top of the free tubulin heterodimer, preventing polymerization. Introduction The microtubule (MT) cytoskeleton plays a wide variety of structural and functional roles in cells, including maintenance of cell shape, intracellular transport, organization of the spatial distribution of organelles in the cytoplasm, cell polarity and chromosome segregation during mitosis (for a review, see Cole and Lippincott-Schwartz, 1995). The characteristic property of MTs, known as dynamic instability, involves rapid transitions between polymerization and depolymerization of α- and β-tubulin heterodimers from the ends of MTs. The MT dynamics are regulated by microtubule-associated proteins (MAPs), which favour MT polymerization, thereby stabilizing the MTs. MAP phosphorylation weakens the stabilizing effect, presumably by decreasing their affinity toward MTs (for a review, see Hirokawa, 1994). However, MAPs alone do not account for the regulation of MT dynamics during the cell cycle. An analysis of MT dynamics in intact cells suggests the presence of MT regulatory factors that oppose the action of MAPs by inducing depolymerization, also termed catastrophe (Drechsel et al., 1992). More recently another type of cellular factor has been identified that destabilizes MTs in vitro and in intact cells (Belmont and Mitchison, 1996). These proteins are encoded by the stathmin gene family (Maucuer et al., 1993). Stathmin/OP18 [also termed oncoprotein 18 (OP18), p19, metablastin and prosolin] is a ubiquitous, well-conserved, cytosolic phosphoprotein. Stathmin/OP18 has been detected in all tissues, the highest levels being found in brain, neurons, testis and leukemic lymphocytes. Expression and phosphorylation are modulated by a diverse number of extracellular signals. The phosphorylation state varies during the cell cycle and peaks during mitosis (Marklund et al., 1993). Stathmin/OP18 is phosphorylated on up to four serine residues by different kinases. The known phosphorylation sites are Ser16, Ser25, Ser38 and Ser63. Stathmin/OP18 has been shown to interact directly with MTs (Belmont and Mitchison, 1996). A complex of one stathmin/OP18 molecule binding two tubulin heterodimers (T2S complex) was detected using analytical ultracentrifugation (Jourdain et al., 1997) and by gel filtration chromatography (Curmi et al., 1994). This interaction is directly dependent on the degree of phosphorylation of stathmin/OP18, where increasing phosphorylation inhibits binding to tubulin (Horwitz et al., 1997; Larsson et al., 1997). Cross-linking of tubulin with stathmin/OP18 phosphorylated in varying combinations at the four phosphorylation sites shows that phosphorylation on Ser16 and Ser63 has the strongest effect on tubulin binding. In conjunction with results from biochemical and genetic experiments, those authors propose a model stating that dual phosphorylation on the cyclin-dependent kinase (CDK) sites Ser25 and Ser38 is required for phosphorylation of Ser16 and/or Ser63, but phosphorylation on the CDK sites alone is not sufficient to downregulate stathmin/OP18 activity. The binding constant of stathmin/OP18 to tubulin is only micromolar (Curmi et al., 1997), which is in agreement with the intracellular concentration of tubulin, which is also in the micromolar range. Two possible mechanisms have been proposed to explain the destabilization of MTs by stathmin/OP18: (i) sequestration of the tubulin heterodimers, and thus depletion of the pool of tubulin available for polymerization (for a recent review, see Andersen, 1999); and (ii) catastrophe stimulation as proposed originally by Belmont and Mitchison (1996). Recently Howell et al. (1999) have tried to distinguish between these mechanisms, and found that stathmin/OP18 has a dual functional activity supporting both mechanisms, which are dependent on the pH. They found tubulin-sequestering and catastrophe-enhancing activity at pH 6.8, and a catastrophe-enhancing but no sequestering activity at pH 7.5. We investigated how stathmin/OP18 and the tubulin heterodimer bind to each other. Stathmin/OP18 is composed of three secondary structure elements, which were expressed separately and assayed for their effect on tubulin polymerization. All three secondary structure elements (truncations) were tested for their capability to cross-link with the tubulin heterodimer in the presence of a chemical cross-linker. In order to determine the site of interaction between the proteins, we analysed the cross-linked and completely proteolysed complex of stathmin/OP18 or its truncations with tubulin by mass spectrometry. We found that the two predicted helices of stathmin/OP18 bind to the region around helix 10 of α-tubulin. In the truncated versions of stathmin/OP18, helix 1 binds to a region between helix 4 and strand 5 of α-tubulin. A structural and functional model is suggested for this interaction. Results Structure prediction The secondary structure prediction method PHD has a success score >70% on average when several related sequences are available (Rost et al., 1994). Prediction for the stathmin/OP18 family indicates that there are three regions in terms of secondary structure: a relatively unstructured N-terminus with a potential polyproline II (PPII) helix at its end, followed by two highly charged helices (helices 1 and 2; Figure 1B). these two α-helices, termed H 1 and H 2, additionally have a coiled-coil-forming potential (COILS; Lupas et al., 1991; Maucuer et al., 1995). The helical content in aqueous solution of monomeric peptides in the absence of tertiary interactions can be estimated by the algorithm AGADIR (Muñoz and Serrano, 1994; Lacroix et al., 1998). AGADIR predicts a strong helical propensity for H 1 (47% helical), while H 2 is predicted to have low helical propensity (17% helical) and the N-terminus is essentially unstructured from the helical point of view (3% helical) (Figure 1A). This indicates that the region corresponding to H 1 will, to a large extent, be populating the α-helical conformation in aqueous solution in the absence of contacts with the rest of the protein, while H 2 will be largely unstructured. Sequence analysis also reveals a possible PPII segment close to the N-terminus of α-helix 1 (Figure 1B). The PPII helix structure is characterized by a repetition every three elements and is induced by the presence of several proline residues in a sequence segment. More importantly, when several proline residues are present the PPII conformation is produced in the absence of tertiary contacts (Williamson, 1994). Figure 1.Secondary structure prediction of stathmin/OP18. Stathmin/OP18 is a 149-amino-acid protein with four consensus serine phosphorylation sites. (A) The secondary structure prediction by PHD predicts a mainly helical protein (dotted line). The N-terminus is relatively unstructured, followed by a long helical stretch interrupted at residues 87–94. Helix calculation by AGADIR predicts a strong helix between residues 46 and 78 only. We have identified a possible PPII helix between the N-terminus and the first helix. The helical region has coiled-coil-forming potential. (B) Model of the secondary structure elements and phosphorylation sites of stathmin/OP18. The protein is divided into three parts: N-terminus, helix 1 and helix 2. Download figure Download PowerPoint Analysis of the proposed polyproline II segment We have investigated the presence of the PPII conformation by comparing a far-UV spectrum of a peptide with the stathmin/OP18 PPII sequence with that of a peptide of the same sequence but with one of the prolines substituted by a glycine, which would disrupt the PPII-typical sequence and conformation. The spectra were recorded under identical conditions at 4°C and corrected for concentration (Figure 2). The spectrum has the typical signature of a PPII conformation, with a maximum around 228 nm and a minimum around 201 nm (Viguera et al., 1994; Pisabarro and Serrano, 1996). The higher intensity signal of the proposed polyproline-containing peptide as compared with the control indicates that this part of stathmin/OP18 can indeed take up a PPII helical conformation. Figure 2.Far-UV CD spectrum of a peptide with the sequence of the proposed PPII helical segment of stathmin/OP18 (continuous line) and a control peptide with the signature sequence destroyed (dotted line). The peptide sequences are YGPEFPLSPP as the original stathmin/OP18 sequence and YGPEFGLSPP as the control. The N-terminal tyrosine was added in order to be able to determine peptide concentration. The PPII-typical signature of the original stathmin/OP18 peptide is much stronger than that of the control, which indicates PPII–like conformation. Download figure Download PowerPoint Cloning, expression and purification Following the secondary structure analysis, we defined three regions in the stathmin/OP18 molecule: the N-terminus (1–43), helix 1 (44–89) and helix 2 (90–142). We cloned each secondary structure element separately as well as the consecutive combinations of N-terminus and helix 1 (N+H 1) and helices 1 and 2 (H 1+H 2). In all cases we were able to express and purify the constructions, except in the case of the N-terminal region alone. CD analysis We compared the CD spectra of stathmin/OP18 and its truncation products (Figure 3B). The whole protein presents a typical α-helical spectrum with a helix content of ∼43% [calculated according to the method of Chen et al. (1974)]. More interestingly, the α-helix content of H 1 (62%) is higher than that of the full protein, while H 2 shows a low helix content (17%). These data agree with the helical tendency predicted by AGADIR. Comparison of the CD spectra of H 1 and H 2 separately with truncation (H 1+H 2) indicates that the combined truncation products have a larger helical content than the simple addition of the individual CD spectra, or AGADIR, would predict (52% helix versus a calculated helicity value assuming additivity of 38%). This could be due to mutual stabilization of the helical content of the two truncation products. Figure 3.Far-UV CD spectra of recombinant human stathmin/OP18 and its truncation products. (A) Thermal melting curve recorded at 222 nm. Stathmin/OP18 (0.1 mg/ml) is in 50 mM sodium phosphate pH 7.0. (B) Mean residue ellipticity of holo-stathmin/OP18 and its truncation products. (C) Theoretical spectrum of the N-terminus calculated by subtracting the spectrum of helix 1 from that of (N+H 1). The resulting spectrum represents a random coil. (D) Theoretical spectrum of the N-terminus calculated by subtracting the spectrum of truncation (H 1+H 2) from that of stathmin/OP18. The resulting spectrum has some helical signal. Download figure Download PowerPoint Since we could not express the N-terminus alone, we calculated its spectrum by subtracting the spectrum of H 1 from that of N+H 1 (Figure 3C). Alternatively, the spectrum of the N-terminus can also be calculated by subtracting the spectra of truncation (H 1+H 2) from that of full-length stathmin/OP18 (Figure 3D). In principle, if the resulting spectra are different this will indicate that the presence of H 2 affects the conformation of H 1. We found that in the first case the result is a random coil spectrum (Figure 3C). This agrees with the 3% helix content predicted by AGADIR for the N-terminus alone, although there is always the question to what extent we can consider that the spectra of the two truncations will be strictly additive. However, this objection does not preclude the comparison with the other subtraction spectrum, since in both cases we always miss the N-terminus. In the case of the comparison between (H 1+H 2) and full-length stathmin/OP18, the resulting spectrum shows some residual helix content (Figure 3D). Therefore, it seems that the presence of H 2 results in a conformational change in the N-terminal region, suggesting the presence of some long-range interactions. Thermal denaturation We measured the thermal denaturation of stathmin/OP18 from 4 to 95°C (Figure 3A). Stathmin/OP18 does not behave as expected for a globular protein. For a folded protein with a distinct tertiary structure, a curve of sigmoidal shape is expected, due to the cooperative nature of protein denaturation. Here, stathmin/OP18 behaves like a peptide, i.e. there is not a single well-defined three-dimensional structure but rather an ensemble of conformations in equilibrium. The lack of cooperative behaviour in stathmin/OP18 is not due to protein aggregation, since the CD spectrum after heating and subsequent cooling is identical to that before heat treatment (data not shown). We know from the literature (Belmont and Mitchison, 1996) and our own assays (data not shown) that the activity of stathmin/OP18 is heat resistant. The poor dispersion of the lines in a one-dimensional NMR spectrum also suggests that the protein is not folded (data not shown). Together these data lead to the conclusion that stathmin/OP18 is not folded in isolation but could take up a defined tertiary structure upon binding to tubulin or other proteins. Effect of stathmin/OP18 and its truncation products on microtubule assembly Spontaneous polymerization of tubulin into MTs was inhibited efficiently by stathmin/OP18 and some of its truncation products. Figure 4 shows that full-length stathmin/OP18 and the truncation comprising helices 1 and 2 (H 1+H 2) were equally efficient in inhibiting tubulin polymerization, followed by the truncation comprising the N-terminus and helix 1 (N+H 1). The helices by themselves had only a very small effect on polymerization in the concentration range studied. The fact that truncation (H 1+H 2) and truncation (N+H 1) are sufficient for inhibiting tubulin polymerization indicates that each subdomain of stathmin/OP18 can interact independently with the tubulin molecule. Figure 4.Inhibition of tubulin polymerization by stathmin/OP18 and its truncation products. Bovine brain tubulin in BRB80 (5.8 mg/ml) and 33% glycerol were polymerized in the presence of different concentrations of stathmin/OP18 or its truncation products at 37°C. The end point of polymerization was recorded at 350 nm. Download figure Download PowerPoint Cross-linking of the tubulin heterodimer and stathmin/OP18 and its truncation products Previous studies have shown that stathmin/OP18 forms mainly a complex with α-tubulin upon chemical cross-linking with the zero-length cross-linker 1-ethyl-3-3(dimethylamino)propylcarbodiimide (EDC) (Larsson et al., 1997; Moreno et al., 1999). N-terminal sequencing of the cross-linked complex indicated that 80–90% of the tubulin cross-linked to stathmin/OP18 is α-tubulin and 10–20% is β-tubulin (Larsson et al., 1997). We studied the binding of the stathmin/OP18 truncation products to the tubulin heterodimer using the same chemical cross-linker. The truncation products were added at the same molar concentration as the full-length stathmin/OP18. As a control, to exclude non-specific cross-linking effects, two proteins that should not interact with tubulin were cross-linked under the same conditions [bovine serum albumin (BSA) and a chemotactic protein from Escherichia coli CheY]. We found that all the stathmin/OP18 fragments tested cross-link to tubulin (Figure 5). Under the same conditions BSA did not cross-link to tubulin, while there was some minor cross-linking to CheY (Figure 5A). In all the cases a band corresponding to the molecular weight of the tubulin dimer was found. A Western blot was probed with an anti-α-tubulin antibody, an anti-β-tubulin antibody and an anti-stathmin/OP18 antibody. All antibodies recognized the cross-linked products (Figure 5B and C, with the exception of H 2, which does not contain the epitope against which the anti-stathmin/OP18 antibody was raised). The fact that the anti-β-tubulin antibody also recognizes the cross-linked products is expected (data not shown) since as was mentioned above 10–20% of the cross-linking of stathmin/OP18 is to β-tubulin. Figure 5.Cross-linking of stathmin/OP18 or its truncation products with tubulin revealed by a zero-length cross-linker. Bovine brain tubulin (11.6 μM) and stathmin/OP18 (or its truncations, 6 μM) were incubated at 4°C for 1 h. The complex was cross-linked with EDC for 30 min at room temperature. The complexes were analysed by Coomassie-stained SDS–PAGE gels or by Western blotting. The positions of α- and β-tubulin and the specific complexes formed are indicated. Positions of the molecular weight standards are given on the left. (A) Coomassie-stained 12% SDS–PAGE gel. (B) Western blot of a 10% SDS–PAGE gel stained with an anti-α-tubulin antibody. (C) Western blot of a 10% SDS–PAGE gel stained with an anti-stathmin/OP18 antibody. Download figure Download PowerPoint Interestingly, additional higher molecular weight products involving stathmin/OP18 and tubulin were only detected in the cross-link of the full-length protein with tubulin. These products may represent the Tα2S complex postulated by Curmi et al. (1997) and Jourdain et al. (1997), and higher order complexes such as T(aβ)2S or T3S (Larsson et al., 1999). The fact that it only occurs with the full-length protein and not with any of the truncations indicates that all elements of stathmin/OP18 are necessary for T2S and higher order complex formation. Determination of stathmin/OP18–α-tubulin binding sites Moreno et al. (1999) have narrowed down the site of interaction between stathmin/OP18 and α-tubulin to the C-terminal residues 307–417. This area comprises residues far away in the three-dimensional structure of tubulin (Nogales et al., 1998) and therefore does not allow the postulation of a model for the inhibition of tubulin polymerization by stathmin/OP18. To define the site of interaction between the two proteins more closely we used an approach involving a combination of mass spectrometry and chemical cross-linking. In short, the previously described EDC-cross-linked tubulin–stathmin/OP18 complex, as well as those complexes between tubulin and stathmin/OP18 truncations, were separated from their constituents and high-molecular-weight by-products by SDS–PAGE and subsequently electroeluted from unstained gel slices. The samples were completely proteolysed by either trypsin (cleaves C-terminal of arginine and lysine) or Endo-LysC (cleaves C-terminal of lysine only). The chemical cross-linker EDC specifically links lysine with either aspartate or glutamate. Stathmin/OP18, its truncation products and tubulin were independently subjected to the same chemical reaction, or left uncross-linked, and digested as controls. The masses of the fragments were determined using MALDI-TOF MS. The controls were used to separate the fragments corresponding to uncross-linked stathmin/OP18/truncations and tubulin, as well as any internal cross-links within these two proteins, from those corresponding to stathmin/OP18/truncations–tubulin cross-linked fragments (Figure 6). The intensity of corresponding peaks in different MALDI mass spectra can vary to a considerable extent owing to the choice of matrix (Cohen and Chait, 1996) and suppression effects. For this reason samples have been analysed from both α-cyano-4-hydroxy-trans-cinnamic acid and 2,5-hydroxybenzoic acid. Only those peaks found in spectra recorded for the cross-linked dimer but not found in any of the spectra recorded for the unmodified or modified monomers were considered as candidates for cross-linked peptides and used for the comparison with the model. Because of the uncertainties associated with interpreting peak heights between different spectra it is essential that cross-link information be deduced only in a statistical manner from a set of candidates obtained from many different measurements. Peaks coinciding with known keratin contaminations have been excluded from the list of candidates. Figure 6.MALDI mass spectra of truncation H 1+H 2, a mixture of α/β-tubulin, and the cross-linked product. Spectra were recorded using α-cyano-4-hydroxy-trans-cinnamic acid as the matrix. The labelled peak corresponds to candidates for cross-linked peptides. Download figure Download PowerPoint To identify the cross-linked sequences a program developed by one of the authors (MASA; L.Serrano) was employed. This program determines the masses of all the fragments produced by a protease in the presence of a specific cross-linking agent, taking into account partial digestion products. It searches for non-cross-linked and all possible inter- and intra-molecule cross-links that will render the experimentally determined mass within a given margin of error. Tubulin purified from mouse brain was used for this study since the sequences of all isomers of α-tubulin from this organism are available from the database, whereas no sequence information is available for the most commonly used bovine brain tubulin. Tubulin isomers are more or less tissue specific, e.g. mouse tubulin α3/7 is specific for testis, whereas all other isomers can be found in different proportions or as trace amounts in different kinds of tissues (Villasante et al., 1986). The major isomers in brain are α1 and α2. The small differences in amino acid sequence (e.g. α1 and α2 differ only at position 232) are detectable with mass spectrometry and it is therefore necessary to take all possible isomers into account in the analysis. The differences between the four known α-isomers encompass only very few amino acids and reside mainly in the 10 C-terminal residues, which are highly postranslationally modified in the cell (Redeker et al., 1994, 1998; Vinh et al., 1999). Apart from the known addition and elimination of a tyrosine at the C-terminus of α-tubulin, the rest of the chemical modifications occurring at the C-terminal regions are quite heterogeneous. Therefore, except for the extra tyrosine residue, we cannot take into consideration these modifications and will miss any cross-linking of stathmin/OP18 to the C-terminus of tubulin. The results from the MALDI-TOF-MS analysis of the digested fragments are shown in Table I. The full-length stathmin/OP18–tubulin cross-links only were additionally digested with Endo-LysC. Table 1. Masses from mass spectroscopy and possible cross-linked fragments calculated by MASA between stathmin/OP18 or its truncations and tubulin Mass from measurement Digestion enzyme Δ to calc. mass Mouse tubulin Stathmin/OP18 1638.85 T 0.08 162–165 84–92 (H1 + H2)h1 0.0 155–163 121–127 (H1 + H2) 1674.77 T 0.11 155–162 69–77 (H1)h1 1870.95 T 0.12 (α4) 325–338 133–136 (H1 + H2)H2 0.1 61–69, 131–136 2765.24 T 0.19 155–165 102–116 0.13 307–319 36–48 0.16 337–351 102–111H2 4368.60 T 0.19 214–242 59–69 0.23 325–335 59–87H1 2196.25 L 0.05 (α4) 325–335 59–69H1 3540.99 L 0.06 325–337 116–135H2 0.03 69–82, 126–142 3591.64 L 0.11 303–325 102–111H2 3606.58 L 0.21 303–325 60–69H1 3623.69 L 0.11 303–310 87–111H2 0.11 50–60, 135–156 T, trypsin digest; L, Endo-LysC digest. The tubulin isomers in the cross-linked fragments are α1 and α2 unless noted otherwise. The numbering of Stathmin/OP18 includes the residues added by the His-tag. Unique fragments are in bold. Fragments used for the model are denoted according to their location on tubulin: H2H2 on the MT outside; H1H1 on MT inside; h1H1 at the 155–165 loop. In the analysis of the full-length stathmin/OP18–tubulin complex nine peaks were identified in the Endo-LysC digest, of which five were matched with possible stathmin/OP18–tubulin cross-links by the program. In the trypsin digest of the full-length stathmin/OP18–tubulin complex 12 peaks were identified, three of which correspond to masses that match common keratin contaminants and two could be matched to possible cross-linked fragments. In total, seven peaks were found that correspond to possible cross-linked fragments of the full-length α-tubulin–stathmin/OP18 complex within the margin of error of the measurement (∼0.01% of the molecular weight measured, 100 p.p.m.). Of these seven fragments, three are unique, corresponding to a single combination of stathmin/OP18 and α-tubulin: α-tubulin 325–335 with 59–69 (H 1) of stathmin/OP18, α-tubulin 303–325 with 60–69 (H 1) of stathmin/OP18 and the same tubulin fragment with region 102–111 (H 2) of stathmin/OP18. In the five remaining cases there is more than one fragment combination fitting the experimental mass. In two of them, one combination explaining the experimental mass involves homo-cross-linking of tubulin, or stathmin/OP18. However, the homo-cross-links of tubulin could be eliminated due to their physical impossibility (data not shown). The analysis of the truncation products cross-linked to tubulin yielded eight possible masses, of which one mass matches a possible keratin contaminant and four masses correspond to possible cross-linked fragments, one of which is unique. This unique fragment identifies H 1 of stathmin/OP18 cross-linked to α1/2-tubulin 155–165. The masses derived from H 2 and N+H 1 yielded no possible cross-links. See Table I and Figures 7 and 8, residue numbers in stathmin/OP18 include the nine residues added by the His-tag. Figure 7.The αβ-tubulin heterodimer (PDB accession code 1TUB). The fragments of α-tubulin that have been found to cross-link to stathmin/OP18 are marked in red. The lysines, aspartates and glutamates within these fragments that could furbish the actual cross-links to stathmin/OP18 are in orange. The fragment 155–165 from α-tubulin is marked in blue [the figure has been generated with MOLSCRIPT (Kraulis, 1991)]. Download figure Download PowerPoint Figure 8.Model for the interaction of stathmin/OP18 with α-tubulin. (A) The truncated forms of stathmin/OP18 bind only to one α-tubulin of the heterodimer. H 1 binds to H4–B5 (155–162) of α-tubulin, a region involved in lateral contacts between filaments. H 2 of stathmin/OP18 binds around helix 10 of α-tubulin. (B) For the complete molecule of stathmin/OP18 binding of H 1 to α-tubulin changes. H 1 binds close to the opposite side of helix 10 of α-tubulin, in such a manner that two heterodimers are connected by one molecule of stathmin/OP18. Download figure Download PowerPoint Discussion Stathmin/OP18 does not have an ordered tertiary structure The data obtained by CD agree with the secondary structure prediction by PHD (Rost et al., 1994) in that the structure of stathmin/OP18 is mainly helical even though there are large differences in the helicity of the two main helices. The spectra obtained from the single components are in