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Preparation of genosensor for detection of specific DNA sequence of the hepatitis B virus

2014; Elsevier BV; Volume: 314; Linguagem: Inglês

10.1016/j.apsusc.2014.06.084

1873-5584

Ana Cristina Honorato de Castro, Erick G. França, Lucas F. de Paula, Márcia Maria Costa Nunes Soares, Luíz Ricardo Goulart, João M. Madurro, Ana G. Brito‐Madurro,

RNA and protein synthesis mechanisms

An electrochemical genosensor was constructed for detection of specific DNA sequence of the hepatitis B virus, based on graphite electrodes modified with poly(4-aminophenol) and incorporating a specific oligonucleotide probe. The modified electrode containing the probe was evaluated by differential pulse voltammetry, before and after incubation with the complementary oligonucleotide target. Detection was performed by monitoring oxidizable DNA bases (direct detection) or using ethidium bromide as indicator of the hybridization process (indirect detection). The device showed a detection limit for the oligonucleotide target of 2.61 nmol L−1. Indirect detection using ethidium bromide was promising in discriminating mismatches, which is a very desirable attribute for detection of disease-related point mutations. In addition, it was possible to observe differences between hybridized and non-hybridized surfaces by atomic force microscopy.