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Microbiological enantioselective reduction of ethyl acetoacetate

2003; Elsevier BV; Volume: 24-25; Linguagem: Inglês

10.1016/s1381-1177(03)00111-5

1873-3158

Joyce Benzaquem Ribeiro, Maria da Conceição Klaus V. Ramos, Francisco Radler de Aquino Neto, Selma Gomes Ferreira Leite, Octávio Augusto Ceva Antunes,

Microbial Metabolic Engineering and Bioproduction

Different microorganisms (MOs) were used to carry out the enantioselective reduction of ethyl acetoacetate to (S)-(+)-3-hydroxybutanoate or (R)-(−)-3-hydroxybutanoate. Surprisingly, the commercially available Saccaromyces cerevisiae led to only 58% ee of (S)-(+)-3-hydroxybutanoate. Other MOs such as Hansenula sp. and Dekera sp. furnished the same enantiomer with greater ees, 81 and 73%, respectively. Aspergilus niger and Kluyveromyces marxianus although leading to lower ees, 30 and 18%, yielded the opposite enantiomer. All reactions proceeded to greater than 85% conversion. This is the first report on the use of Hansenula sp. and Dekera sp. in reductions of β-ketoesters.