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Prevalence of Actinobacillus actinomycetemcomitans serotypes in Japanese patients with periodontitis

1997; Wiley; Volume: 32; Issue: 8 Linguagem: Inglês

10.1111/j.1600-0765.1997.tb00578.x

1600-0765

Matuo Yamamoto, Tatsuji Nishihara, Takeyoshi Koseki, Tao He, Kenji Yamato, Yijie Zhang, Keisuke Nakashima, Shigeru Oda, Isao Ishikawa,

Microbial infections and disease research

Oral Actinobacillus actinomycetemcomitans strains are serologically classified into 5 distinct groups, a to e. We examined the distribution of A. actinomycetemcomitans serotypes in Japanese patients with periodontitis. A total of 157 A. actinomycetemcomitans clinical isolates from diseased sites of 39 patients with periodontitis were serotyped by using serotype‐specific rabbit antisera against. A. actinomycetemcomitans serotypes a, b, c, d and e strains. In the immunodiffusion assay, autoclaved extracts of 42, 6, 39, 9 and 41 A. actinomycetemcomitans clinical isolates reacted with serotypes a, b, c, d and e antisera, respectively. Although 37 patients were infected with a serotype strain, 2 patients harbored 2 different serotype strains, b/e and b/untypeable. To establish a correlation between serotype and genotype of A. actinomycetemcomitans clinical isolates from 2 patients who had different serotype strains, we used arbitrarily primed polymerase chain reaction (AP‐PCR) to fingerprint clinical isolates of different serotypes. The AP‐PCR genotype among 4 clinical isolates (b/e and b/untypeable) were identical to that of A. actinomycetecomitans Y4 (serotype b), indicating the presence of multiple A. actinomycetemcomitans serotypes which are genetically homogenous in the periodontally diseased sites of patients with periodontitis.