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Simultaneous quantitative determination of metronidazole and nalidixic acid in tablets by difference spectroscopy

1996; Elsevier BV; Volume: 14; Issue: 4 Linguagem: Inglês

10.1016/0731-7085(95)01659-7

1873-264X

P. Parimoo, C. V. N. Prasad, R. Vineeth,

Antibiotics Pharmacokinetics and Efficacy

A difference spectrophotometric procedure has been developed for simultaneous determination of metronidazole (MDZ) and nalidixic acid (NA) in tablets. The method comprised the measurement of the absorbance of a solution of the tablet extract in 0.1 M NaOH relative to that of an equimolar solution in 0.1 M HCl at 292 nm for NA and 325 nm for MDZ. The presence of identical isosbestic points for pure drug solutions and tablet extracts indicated the non-interference of excipients in the absorption at these wavelengths. Compliance with Beer's law was observed in the concentration ranges 5-25 micrograms ml(-1) for MDZ and 15-35 micrograms ml(-1) for NA these wavelengths.