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An easy, quick and cheap high-throughput method for yeast DNA extraction from microwell plates

2013; Elsevier BV; Volume: 93; Issue: 3 Linguagem: Inglês

10.1016/j.mimet.2013.03.016

1872-8359

João Drumonde‐Neves, E. Vieira, Maria Thereza Alves De Lima, Isabel Inês Araújo, Margarida Casal, Dorit Elisabeth Schuller,

Fungal and yeast genetics research

We herein present a high-throughput and cheap method for yeast DNA isolation in a 96 well microplate. About1500 yeast isolates can be processed within one working day and final DNA concentrations are suitable for direct application in PCR-based molecular typing methods.