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Regeneration of 2,3‐bisphosphoglycerate and ATP in stored erythrocytes by phosphoenolpyruvate: a new preservative for blood storage

1981; Wiley; Volume: 21; Issue: 4 Linguagem: Inglês

10.1046/j.1537-2995.1981.21481275994.x

1537-2995

Naotaka Hamasaki, Hiroshi Ideguchi, Yuzuru Ikehara,

Blood donation and transfusion practices

Phosphoenolpyruvate is transported through erythrocyte membrane in acid citrate or sucrose solution and metabolized to 2,3‐bisphosphoglycerate and pyruvate. The levels of ATP and 2,3‐bisphosphoglycerate of depleted, stored erythrocytes were increased effectively by incubating the cells in acid‐citrate‐dextrose‐sucrose solution with phosphoenolpyruvate. The concentration of 2,3‐bisphosphoglycerate exceeded by three times its physlological level under suitable conditions. When fresh blood was stored at 4 C in acid‐citrate‐dextrose‐sucrose solution with phosphoenolpyruvate, the levels of ATP and 2,3‐bisphosphoglycerate of erythrocytes decreased during storage in essentially the same extent as those of erythrocytes preserved in acid‐citrate‐dextrose solution. However, the levels of ATP and 2,3‐bisphosphoglycerate of the cells increased when preserved erythrocytes with phosphoenolpyruvate were incubated at 37 C for 30 minutes. Especially noteworthy was the ATP concentration of erythrocytes preserved with phosphoenolpyruvate at 4 C for 14 days. It was kept at the same level as in fresh blood by the short incubation time.