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Allergenicity and cross-reactivity of rye grass pollen extracts revealed by monoclonal antibodies

1986; Elsevier BV; Volume: 89; Issue: 1 Linguagem: Inglês

10.1016/0022-1759(86)90031-1

1872-7905

Walid Mourad, Guy Pelletier, André Boulet, Md. Nazrul Islam, Jean‐Paul Valet, Jacques Hébert,

Toxin Mechanisms and Immunotoxins

Using monoclonal antibodies the immunogenic and allergenic characteristics of rye Group I were redefined by SDS-PAGE analysis and immunoblotting. The purified rye Group I from NIH possesses a major component of approximately 34 000 Da against which most of the sera from grass-sensitive patients and none from non-atopic volunteers contain specific IgE antibodies. Monoclonal antibodies.to rye Group I were raised and used to purify the antigen and to verify the cross-reactivity between grass extracts. The 3 monoclonal antibodies studied recognized different components of timothy grass and 2 of them recognized kentucky june grass but none recognized components of ragweed extracts.