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Somatic embryogenesis and plant regeneration in Opuntia ficus-indica (L.) Mill. (Cactaceae)

2006; Elsevier BV; Volume: 108; Issue: 1 Linguagem: Inglês

10.1016/j.scienta.2005.12.007

1879-1018

Francisco L.A.F. Gomes, Fabíola Fernandes Heredia, Priscilla Barbeta e Silva, Olivardo Facó, Francisco Campos,

Seed Germination and Physiology

We present here a method for the regeneration of the prickly-pear (Opuntia ficus-indica (L.) Mill.) through somatic embryogenesis (SE). Direct SE was induced by cultivating shoot apices devoid of leaf primordia on semisolid Murashige and Skoog (MS) basal medium supplemented with 4-amino 3,5,6-trichloropicolinic acid (picloram) at 4 mg l−1. Somatic embryogenesis was influenced by the type, age, physiological and developmental stage of the explants, and also by light conditions, wounding, and the sucrose concentration in the induction medium. A histological analysis confirmed SE by revealing the presence of a closed vascular system in the developing embryos and the absence of a vascular connection between the somatic embryos and the explant.