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Characterization of a Human TAR RNA-Binding Protein That Activates the HIV-1 LTR

1991; American Association for the Advancement of Science; Volume: 251; Issue: 5001 Linguagem: Inglês

10.1126/science.2011739

1095-9203

Anne Gatignol, Alicia Buckler‐White, Ben Berkhout, Kuan‐Teh Jeang,

Viral Infections and Immunology Research

Human immunodeficiency virus type 1 (HIV-1) gene expression is activated by Tat, a virally encoded protein. Tat trans-activation requires viral (trans-activation—responsive; TAR) RNA sequences located in the R region of the long terminal repeat (LTR). Existing evidence suggests that Tat probably cooperates with cellular factors that bind to TAR RNA in the overall trans-activation process. A HeLa complementary DNA was isolated and characterized that encodes a TAR RNA-binding protein (TRBP). TRBP activated the HIV-1 LTR and was synergistic with Tat function.