Parallel Nanoliter Microfluidic Analysis System
2007; American Chemical Society; Volume: 79; Issue: 11 Linguagem: Inglês
10.1021/ac061692y
ISSN1520-6882
AutoresPer Andersson, Gérald Jesson, Gunnar Kylberg, Gunnar Ekstrand, Gunnar Thorsén,
Tópico(s)Innovative Microfluidic and Catalytic Techniques Innovation
ResumoA parallel nanoliter microfluidic analysis system based on capillary action, centrifugal force, and hydrophobic barriers is described. The precision of 112 parallel volume definition operations is determined to 0.75% CV at 200 nL using the individual sample introduction structure. For 20 nL, the actual measurement error is the dominating factor, with a combined error of 1.9% CV. Individual dispensing as well as dispensing through a common distribution channel is described. The volume definition precision for the common distribution channel is 1.6% CV for 200 nL. Unlike the dominating forces in microliter-sized channel systems, we describe hysteresis effects as exerting a major influence, which needs to be considered in order to control the operation and design of discrete nanoliter fluidics. Hydrophobic patches at the corners of the rectangular channel control corner-enhanced wicking. Excellent flow control of 1 and 2 nL/s is achieved using a predefined spin program.
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