Açaí (Euterpe oleraceae Mart.) berry extract exerts neuroprotective effects against β-amyloid exposure in vitro
2013; Elsevier BV; Volume: 556; Linguagem: Inglês
10.1016/j.neulet.2013.10.027
ISSN1872-7972
AutoresDaphne Yiu San Wong, Ian F. Musgrave, Benjamin S. Harvey, Scott D. Smid,
Tópico(s)Parkinson's Disease Mechanisms and Treatments
ResumoThe native South American palm açaí berry (Euterpe oleraceae Mart.) has high polyphenolic and antioxidant levels. This study examined whether açaí berry extract afforded protection against β-amyloid (Aβ)-mediated loss of cell viability and oxidative stress associated with anti-fibrillar effects. PC12 cells were exposed to either Aβ1–42, Aβ25–35 or tert butyl hydroperoxide (t-BHP), alone or in the presence of açaí extract (0.5–50 μg/ml). Thioflavin T (ThT) binding assay and transmission electron microscopy were used to determine effects of açaí extract on Aβ1–42 fibril morphology and compared to açaí phenolics gallic acid, cyanidin rutinoside and cyanidin glucoside. Exposure to Aβ1–42, Aβ25–35 or t-BHP decreased PC12 cell viability. Pretreatment with açaí extract significantly improved cell viability following Aβ1–42 exposure, however Aβ25–35 or t-BHP-mediated viability loss was unaltered. Açaí extract inhibited ThT fluorescence and disrupted Aβ1–42 fibril and aggregate morphology. In comparison with other phenolics, açaí was most effective at inhibiting Aβ1–42 aggregation. Inhibition of β-amyloid aggregation may underlie a neuroprotective effect of açaí.
Referência(s)