The difference between ADP‐beryllium fluoride and ADP‐aluminium fluoride complexes of the spin‐labeled myosin subfragment 1
1995; Wiley; Volume: 371; Issue: 3 Linguagem: Inglês
10.1016/0014-5793(95)00898-j
ISSN1873-3468
AutoresMichael A. Ponomarev, Vladimir P. Timofeev, Dmitrii I. Levitsky,
Tópico(s)Advanced MRI Techniques and Applications
ResumoElectron paramagnetic resonance (EPR) spectroscopy was used for investigation of the structure of spin‐labeled myosin subfragment 1 (S1) containing ADP and phosphate analogues, such as orthovanadate, aluminium fluoride (AlF 4 ), and beryllium fluoride (BeF x ). It has been shown that the local conformational changes in the region of Cys‐707, induced by formation of the S1‐ADP‐BeF x complex, differ from those of S1 containing ADP‐AlF 4 or other phosphate analogues but are similar to the changes which occur in the presence of ADP or ATPγS. It is suggested that S1‐ADP‐AlF 4 and S1‐ADP‐BeF x complexes represent structural analogues of different transition states of the ATPase cycle, namely the intermediate states S1 ** ‐ADP‐P i and S1 * ‐ATP, respectively.
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