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Multidrug-resistant tet(X)-containing hospital isolates in Sierra Leone

2013; Elsevier BV; Volume: 42; Issue: 1 Linguagem: Inglês

10.1016/j.ijantimicag.2013.04.014

1872-7913

Tomasz A. Łęski, Umaru Bangura, David Henry Jimmy, Rashid Ansumana, Stephen E. Lizewski, David A. Stenger, Chris R. Taitt, Gary J. Vora,

Vibrio bacteria research studies

The tet(X) gene encodes a flavin-dependent monooxygenase that confers resistance to all clinically relevant tetracycline antibiotics including tigecycline. It has only previously been identified in environmental and non-human pathogenic bacteria. To investigate levels of multidrug resistance in Bo, Sierra Leone, a molecular epidemiological study was conducted using an antimicrobial resistance determinant microarray (ARDM), PCR and DNA sequencing. The study found that 21% of isolates from Mercy Hospital (Bo, Sierra Leone) were tet(X)-positive, all of which originated from urinary specimens. Use of molecular epidemiological surveillance tools has provided the first evidence of tet(X)-containing multidrug-resistant Gram-negative hospital isolates in a hospital in Sierra Leone.