Versatile transformation vectors to assay the promoter activity of DNA elements in plants
1994; Elsevier BV; Volume: 149; Issue: 2 Linguagem: Inglês
10.1016/0378-1119(94)90179-1
ISSN1879-0038
AutoresGiancarlo Pasquali, Pieter B. F. Ouwerkerk, Johan Memelink,
Tópico(s)Plant Reproductive Biology
ResumoA convenient vector system was developed to evaluate transcriptional promoter activities in plants. Two primary vectors, optionally containing the cauliflower mosaic virus (CaMV) 35S -47 or -90 minimal promoters, offer multiple sites for cloning the sequence of interest upstream from the beta-glucuronidase gene (gusA). The promoter-gusA cassette can be transferred to a binary vector containing the selectable neomycin phosphotransferase II-encoding gene (nptII) next to the left border. In addition, the transferred DNA (T-DNA) contains the chloramphenicol acetyltransferase gene (cat) driven by the CaMV 35S promoter. Activity of cat can serve as a reference for gusA expression to correct for effect of chromosomal position or T-DNA copy number.
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