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VASECTOMY IMPAIRS SPERMATOGENESIS THROUGH GERM CELL APOPTOSIS MEDIATED BY THE p53-BAX PATHWAY IN RATS

2001; Lippincott Williams & Wilkins; Volume: 166; Issue: 4 Linguagem: Inglês

10.1016/s0022-5347(05)65831-4

1527-3792

Koji Shiraishi, Katsusuke Naito, Kenichi Yoshida,

DNA Repair Mechanisms

No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Oct 2001VASECTOMY IMPAIRS SPERMATOGENESIS THROUGH GERM CELL APOPTOSIS MEDIATED BY THE p53-BAX PATHWAY IN RATS KOJI SHIRAISHI, KATSUSUKE NAITO, and KEN-ICHI YOSHIDA KOJI SHIRAISHIKOJI SHIRAISHI , KATSUSUKE NAITOKATSUSUKE NAITO , and KEN-ICHI YOSHIDAKEN-ICHI YOSHIDA View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)65831-4AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: The pathophysiology of impaired spermatogenesis after vasectomy has not been completely investigated. We examined the role of p53 protein in cell cycle arrest and apoptosis of germ cells after vasectomy in the rat. Materials and Methods: Eight-week old rats underwent bilateral vasectomy and the testes were harvested 1, 4, 8, 12 and 24 weeks after surgery. Germ cell apoptosis was evaluated by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling (TUNEL) and electrophoresis assay of DNA fragmentation. Western blot analysis and immunohistochemistry were performed to examine the expression of p53, proliferating cell nuclear antigen, Bax, Bcl-2, p21WAF1/Cip1 and Gadd45. To evaluate spermatogenesis, testicular weight and percent of haploid cells flow cytometry was done. Results: Spermatogenesis impairment was associated with increased p53 and decreased proliferating cell nuclear antigen expression at the delayed phase more than 8 weeks after vasectomy. The number of TUNEL positive germ cells was increased at the early 1-week and delayed phases after vasectomy. Bax but not p21WAF1/Cip1 or Gadd45 expression was increased. p53, Bax and TUNEL positive cells were co-localized in the seminiferous tubules. Conclusions: Spermatogenesis was impaired after vasectomy by apoptosis but not by cell cycle arrest. 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Google Scholar From the Department of Urology, Yamaguchi University School of Medicine, Yamaguchi and Department of Forensic Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, Japan© 2001 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byLei B, Wan B, Peng J, Yang Y, Lv D, Zhou X, Shu F, Li F, Zhong L, Wu H and Mao X (2018) PRPS2 Expression Correlates with Sertoli-Cell Only Syndrome and Inhibits the Apoptosis of TM4 Sertoli CellsJournal of Urology, VOL. 194, NO. 5, (1491-1497), Online publication date: 1-Nov-2015.Shiraishi K and Naito K (2018) Effects of 4-Hydroxy-2-Nonenal, a Marker of Oxidative Stress, on Spermatogenesis and Expression of p53 Protein in Male InfertilityJournal of Urology, VOL. 178, NO. 3, (1012-1017), Online publication date: 1-Sep-2007.Shiraishi K, Yoshida K, Fujimiya T and Naito K (2018) Activation of Mitogen Activated Protein Kinases and Apoptosis of Germ Cells After Vasectomy in The RatJournal of Urology, VOL. 168, NO. 3, (1273-1278), Online publication date: 1-Sep-2002. Volume 166Issue 4October 2001Page: 1565-1571 Advertisement Copyright & Permissions© 2001 by American Urological Association, Inc.Keywordstestisspermatogenesisvasectomyprotein p53rats, WistarMetrics Author Information KOJI SHIRAISHI More articles by this author KATSUSUKE NAITO More articles by this author KEN-ICHI YOSHIDA More articles by this author Expand All Advertisement PDF downloadLoading ...