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Tenacibaculum sp. associated with winter ulcers in sea-reared Atlantic salmon Salmo salar

2010; Inter-Research; Volume: 94; Issue: 3 Linguagem: Inglês

10.3354/dao02324

1616-1580

AB Olsen, Hanne Nilsen, Nina Sandlund, Helga Mikkelsen, Henning Sørum, DJ Colquhoun,

Myxozoan Parasites in Aquatic Species

DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 94:189-199 (2011) - DOI: https://doi.org/10.3354/dao02324 Tenacibaculum sp. associated with winter ulcers in sea-reared Atlantic salmon Salmo salar A. B. Olsen1,*, H. Nilsen1, N. Sandlund2, H. Mikkelsen3, H. Sørum4, D. J. Colquhoun5 1National Veterinary Institute Bergen, 5811 Bergen, Norway 2Institute of Marine Research, 5817 Bergen, Norway 3Nofima Marin, 9291 Tromsø, Norway 4Norwegian College of Veterinary Medicine, 0033 Oslo, Norway 5National Veterinary Institute Oslo, 0106 Oslo, Norway *Email: anne-berit.olsen@vetinst.no ABSTRACT: Coldwater-associated ulcers, i.e. winter ulcers, in seawater-reared Atlantic salmon Salmo salar L. have been reported in Norway since the late 1980s, and Moritella viscosa has been established as an important factor in the pathogenesis of this condition. As routine histopathological examination of winter ulcer cases in our laboratory revealed frequent presence in ulcers of long, slender rods clearly different from M. viscosa, a closer study focusing on these bacteria was conducted. Field cases of winter ulcers during 2 sampling periods, 1996 and 2004–2005, were investigated and long, slender rods were observed by histopathological examination in 70 and 62.5% of the ulcers examined, respectively, whereas cultivation on marine agar resulted in the isolation of yellow-­pigmented colonies with long rods from 3 and 13% of the ulcers only. The isolates could be separated into 2 groups, both identified as belonging to the genus Tenacibaculum based on phenotypic characterization and 16S rRNA sequencing. Bath challenge for 7 h confirmed the ability of Group 1 bacterium to produce skin and cornea ulcers. In fish already suffering from M. viscosa-induced ulcers, co-infection with the Group 1 bacterium was established within 1 h. Ulcers from field cases of winter ulcers and from the transmission experiments tested positive by immunohistochemistry with polyclonal antiserum against the Group 1 bacterium but not the Group 2 bacterium. Our results strongly indicate the importance of the Group 1 bacterium in the pathogenesis of winter ulcers in Norway. The bacterium is difficult to isolate and is therefore likely to be underdiagnosed based on cultivation only. KEY WORDS: Winter ulcers · Tenacibaculum · Atlantic salmon · Transmission experiment · Moritella viscosa · Cod · Halibut Full text in pdf format PreviousNextCite this article as: Olsen AB, Nilsen H, Sandlund N, Mikkelsen H, Sørum H, Colquhoun DJ (2011) Tenacibaculum sp. associated with winter ulcers in sea-reared Atlantic salmon Salmo salar. Dis Aquat Org 94:189-199. https://doi.org/10.3354/dao02324 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 94, No. 3. Online publication date: May 09, 2011 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2011 Inter-Research.