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BIBTEX RIS

Sex determination of murine and bovine embryos using cytotoxicity and immunofluorescence assays

1993; Elsevier BV; Volume: 39; Issue: 6 Linguagem: Inglês

10.1016/0093-691x(93)90236-x

1879-3231

Vera Fernanda Martins Hossepian de Lima, Carlos Alberto Moreira‐Filho, A.R. De, W. Jorge,

CRISPR and Genetic Engineering

Murine and bovine embryos were sexed by using polyclonal and monoclonal anti-H-Y antibodies in cytotoxic and immunofluorescence assays. The specificity of 3 different sources of antibodies are compared. Sexing was accomplished by complement-mediated cytotoxicity or by H-Y antigen detection using a anti-mouse Ig fluorescein conjugate. Cytogenetic ascertainment for correct sex identification yielded a score of about 85% in both species, while the immunofluorescence assay yielded a score of 83%.