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Combined scanning optical coherence and two-photon-excited fluorescence microscopy

1999; Optica Publishing Group; Volume: 24; Issue: 14 Linguagem: Inglês

10.1364/ol.24.000969

1539-4794

Emmanuel Beaurepaire, Laurent Moreaux, François Amblard, Jérôme Mertz,

Biochemical and Structural Characterization

We demonstrate simultaneous imaging by optical coherence microscopy (OCM) and two-photon-excited (TPE) fluorescence microscopy. A mode-locked Ti:sapphire laser is focused and scanned in three dimensions through a fixed sample, generating both backscattered light and fluorescence light, which are independently detected. Both imaging modes provide rapid en-face imaging with submicrometer resolution. High-power delivery into the sample yields an OCM sensitivity in excess of 130 dB at 100-kHz pixel rates. Simultaneous imaging of cell nuclei with OCM and TPE is demonstrated in live drosophila embryos.