Immunological relationships between the subunits of textilotoxin and rabbit antisera raised against textilotoxin and some snake venoms
1991; Elsevier BV; Volume: 29; Issue: 3 Linguagem: Inglês
10.1016/0041-0101(91)90291-x
ISSN1879-3150
AutoresJudith A. Pearson, Margaret I. Tyler, M.E.H. Howden,
Tópico(s)Ion channel regulation and function
ResumoThe binding of textilotoxin and its subunits A, B, C and D to polyclonal rabbit antisera directed against textilotoxin, Australian common brown snake (Pseudonaja textilis) and tiger snake (Notechis scutatus scutatus) venoms was studied by ELISA. Subunit D showed greatest reactivity with antisera to textilotoxin and brown snake venom. The phospholipase A2 active subunit A reacted more strongly with antisera to tiger snake venom in keeping with the high degree of homology between the amino acid sequences of subunit A and notexin from tiger snake venom. Subunit A was the only subunit lethal to mice, but at doses 1000-fold greater than for textilotoxin.
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