Characterization of hepatitis B virus (Dane particle)
1980; Elsevier BV; Volume: 1; Issue: 1 Linguagem: Inglês
10.1016/0166-0934(80)90006-3
ISSN1879-0984
AutoresJ. Wai‐Kuo Shih, Geoŗge Hess, Paul M. Kaplan, John L. Gerin,
Tópico(s)Hepatitis C virus research
ResumoAbstract Hepatitis B virions (Dane particles) were purified from the sera of chronic HBsAg carriers by consecutive rate-zonal and isopycnic centrifugations in sucrose gradients using HBsAg, HBcAg and endogenous DNA polymerase activities as specific markers. Purified Dane particles, radiolabelled with Na 125I by the chloramine-T procedure, had a higher buoyant density in CsCl (1.28 g/cm3) than unlabelled particles (1.26 g/cm3) and an estimated sedimentation coefficient of 280 s. 125I-Dane particles were fully precipitated by anti-HBs and not by anti-HBc sera. Heavy and light density core particles were purified from heavy and light density populations of Dane particles and radioiodinated. The iodinated polypeptides of Dane particles and HBcAg were compared with those of the iodinated 22-nm form of HBsAg by SDS-PAGE. Iodinated Dane particles contained seven polypeptides with molecular weights of 18,000, 23,000, 26,000, 34,000, 43,000, 48,000 and 115,000. Heavy and light core particles contained three polypeptides with molecular weights of 18,000, 25,000 and 37,000.
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