Regulation of PCR efficiency with magnetic nanoparticles in a rotating magnetic field
2011; Elsevier BV; Volume: 506; Issue: 4-6 Linguagem: Inglês
10.1016/j.cplett.2011.03.017
ISSN1873-4448
AutoresToshiaki Higashi, Yutaka Nagaoka, Hiroaki Minegishi, Akinobu Echigo, Ron Usami, Toru Maekawa, Tatsuro Hanajiri,
Tópico(s)Microfluidic and Bio-sensing Technologies
ResumoThe polymerase chain reaction (PCR) method is widely used for the reproduction and amplification of specific DNA segments in vitro, and a novel PCR method using nanomaterials such as gold nanoparticles has recently been reported. This paper reports on the regulation of PCR efficiency with superparamagnetic nanoparticles in a rotating magnetic field. The level of efficiency was successfully regulated in a rotating magnetic field by the authors, and decreased with increasing frequency of the field. The results obtained show that simply controlling the structure and dynamics of magnetic nanoparticle clusters in a rotating magnetic field can regulate PCR efficiency.
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