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Anti-inflammatory activity of bark of Dioscorea batatas DECNE through the inhibition of iNOS and COX-2 expressions in RAW264.7 cells via NF-κB and ERK1/2 inactivation

2010; Elsevier BV; Volume: 48; Issue: 11 Linguagem: Inglês

10.1016/j.fct.2010.07.048

1873-6351

Meihua Jin, Seok‐Jong Suh, Ju Yang, Yue Lu, Su Jeong Kim, Soonyoul Kwon, Tae Hyung Jo, Jin Wan Kim, Young In Park, Ghe Whan Ahn, Chong‐Kil Lee, Cheorl‐Ho Kim, Jong‐Keun Son, Kun Ho Son, Hyeun Wook Chang,

Natural product bioactivities and synthesis

We identified a bioactive herbal medicine with anti-inflammatory activity from an ethanol extract derived from the bark of Dioscorea batatas DECNE (BDB) in RAW264.7 cells. We examined the effects of BDB on nitric oxide (NO) and prostaglandin E2 (PGE2) production in LPS-induced RAW264.7 cells. BDB consistently inhibited both NO and PGE2 production in a dose-dependent manner, with an IC50 of 87–71 μg/ml, respectively. The reduction of NO and PGE2 production were accompanied by a reduction in iNOS and COX-2 protein expression, as evaluated by Western blotting. To evaluate the action mode of BDB and its ability to inhibit iNOS and COX-2 protein expression, we assessed the effects of BDB on nuclear factor-κB (NF-κB) DNA-binding activity, NF-κB-dependent reporter gene activity, inhibitory factor-κB (IκB) phosphorylation and degradation, and p65 nuclear translocation. BDB suppressed DNA-binding activity and reporter gene activity as well as translocation of the NF-κB p65 subunit. BDB also down-regulated IκB kinase (IKK), thus inhibiting LPS-induced both phosphorylation and the degradation of IκBα. In addition, BDB also inhibited the LPS-induced activation of ERK1/2.