Portal de Periódicos da CAPES

Comparison of Etest with Modified Broth Microdilution Method for Testing Susceptibility of Aspergillus spp. to Voriconazole

2003; American Society for Microbiology; Volume: 41; Issue: 11 Linguagem: Inglês

10.1128/jcm.41.11.5270-5272.2003

1098-660X

M. C. López Serrano, D. Morilla, Anastasio Valverde, Mónica Chávez, Ana Espinel‐Ingroff, Rosa María Claro, M. Ramírez, Estrella Martín‐Mazuelos,

Infectious Diseases and Mycology

ABSTRACT We compared the Etest with a broth microdilution method, performed according to a modified National Committee for Clinical Laboratory Standards guideline (M38-A), for determining the in vitro susceptibility of 77 isolates of Aspergillus spp. (26 A. fumigatus , 21 A. flavus , 10 A. terreus , 9 A. niger , 5 A. nidulellus , 4 A. glaucus , and 2 A. flavipes isolates). Overall, there was 92.2% agreement between both methods when Etest MICs were read at 24 h and 83.1% agreement when both methods were read at 48 h. When Etest MICs were read at 24 h, the agreement was >90% for all species tested except for A. fumigatus (84.6%). When Etest MICs were read at 48 h, the agreement ranged from 50 to 100%. The poorest agreement was seen with A. glaucus (50%) and A. fumigatus (65%). Where a discrepancy was observed between Etest and the reference method, the Etest MIC was generally higher. The Etest appears to be a suitable alternative procedure for testing the susceptibility of Aspergillus spp. to voriconazole.