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TEM and FISH studies in sperm from men of couples with recurrent pregnancy loss

2009; Wiley; Volume: 41; Issue: 6 Linguagem: Inglês

10.1111/j.1439-0272.2009.00936.x

1439-0272

Giulia Collodel, V. Giannerini, Nicola Antonio Pascarelli, M. G. Federico, F. Comodo, Elena Moretti,

Sperm and Testicular Function

AndrologiaVolume 41, Issue 6 p. 352-360 TEM and FISH studies in sperm from men of couples with recurrent pregnancy loss G. Collodel, G. Collodel Interdepartmental Centre for Research and Therapy of Male Infertility, University of Siena, Siena, ItalySearch for more papers by this authorV. Giannerini, V. Giannerini Department of Biomedical Sciences, Applied Biology Section, University of Siena, Siena, ItalySearch for more papers by this authorN. Antonio Pascarelli, N. Antonio Pascarelli Department of Biomedical Sciences, Applied Biology Section, University of Siena, Siena, ItalySearch for more papers by this authorM. G. Federico, M. G. Federico Department of Biomedical Sciences, Applied Biology Section, University of Siena, Siena, ItalySearch for more papers by this authorF. Comodo, F. Comodo Aleteia PMA viale Vittorio Veneto, Siena, ItalySearch for more papers by this authorE. Moretti, E. Moretti Interdepartmental Centre for Research and Therapy of Male Infertility, University of Siena, Siena, ItalySearch for more papers by this author G. Collodel, G. Collodel Interdepartmental Centre for Research and Therapy of Male Infertility, University of Siena, Siena, ItalySearch for more papers by this authorV. Giannerini, V. Giannerini Department of Biomedical Sciences, Applied Biology Section, University of Siena, Siena, ItalySearch for more papers by this authorN. Antonio Pascarelli, N. Antonio Pascarelli Department of Biomedical Sciences, Applied Biology Section, University of Siena, Siena, ItalySearch for more papers by this authorM. G. Federico, M. G. Federico Department of Biomedical Sciences, Applied Biology Section, University of Siena, Siena, ItalySearch for more papers by this authorF. Comodo, F. Comodo Aleteia PMA viale Vittorio Veneto, Siena, ItalySearch for more papers by this authorE. Moretti, E. Moretti Interdepartmental Centre for Research and Therapy of Male Infertility, University of Siena, Siena, ItalySearch for more papers by this author First published: 05 November 2009 https://doi.org/10.1111/j.1439-0272.2009.00936.xCitations: 12 Elena Moretti, Department of Biomedical Sciences, Applied Biology Section, University of Siena, Policlinico Le Scotte, Viale Bracci, 14, 53100 Siena, Italy. Tel.: +39 0577 233539; Fax: +39 0577 233527; E-mail: [email protected] Research funded by Piano di Ateneo per la Ricerca (PAR grant) 2005, University of Siena, Italy Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Summary The role of the male partner in recurrent pregnancy loss (RPL) is not clear. In this study, semen characteristics of 22 men whose partners had experienced RPL were examined by light microscopy. Sperm morphology was analysed by transmission electron microscopy (TEM) and the data were mathematically elaborated to obtain numerical indices expressing the status of an ejaculate: the fertility index and the percentage of apoptosis, necrosis and immaturity. Sperm apoptosis and necrosis were also evaluated by annexin V/propidium iodide assay. To explore the status of meiotic segregation, fluorescence in situ hybridisation (FISH) with probes for chromosomes 18, X and Y, was applied directly on sperm nuclei. Sperm characteristics from a group of men of proven fertility were used as controls. Among the considered sperm characteristics, apoptosis (P < 0.01), 1818YY diploidy (P < 0.05) and 18YY disomy (P < 0.01) scores were significantly higher in men with RPL compared with controls. Our study showed that some patients with normal semen parameters can present a slight increase in aneuploidy compared with controls, indicating a possible involvement of sperm in some cases of RPL. Chromosomal FISH analysis and chromatin tests of sperm could be included in RPL work-ups when no other cause has been detected. Citing Literature Volume41, Issue6December 2009Pages 352-360 RelatedInformation