Portal de Periódicos da CAPES

Revisiting the Role of the Mother Centriole in Centriole Biogenesis

2007; American Association for the Advancement of Science; Volume: 316; Issue: 5827 Linguagem: Inglês

10.1126/science.1142950

1095-9203

Ana Rodrigues-Martins, Maria Giovanna Riparbelli, Giuliano Callaini, David M. Glover, Mónica Bettencourt‐Dias,

Ubiquitin and proteasome pathways

Centrioles duplicate once in each cell division cycle through so-called templated or canonical duplication. SAK, also called PLK4 (SAK/PLK4), a kinase implicated in tumor development, is an upstream regulator of canonical biogenesis necessary for centriole formation. We found that overexpression of SAK/PLK4 could induce amplification of centrioles in Drosophila embryos and their de novo formation in unfertilized eggs. Both processes required the activity of DSAS-6 and DSAS-4, two molecules required for canonical duplication. Thus, centriole biogenesis is a template-free self-assembly process triggered and regulated by molecules that ordinarily associate with the existing centriole. The mother centriole is not a bona fide template but a platform for a set of regulatory molecules that catalyzes and regulates daughter centriole assembly.