Bioimaging TOF‐SIMS: localization of cholesterol in rat kidney sections
2004; Wiley; Volume: 566; Issue: 1-3 Linguagem: Inglês
10.1016/j.febslet.2004.04.052
ISSN1873-3468
AutoresHåkan Nygren, Per Malmberg, C. Kriegeskotte, Heinrich F. Arlinghaus,
Tópico(s)Analytical chemistry methods development
ResumoRat kidneys were sectioned by cryoultramicrotomy and dried at room temperature. The samples were covered with a thin silver layer and analyzed in an imaging TOF‐SIMS instrument equipped with a Ga + ‐source. The cholesterol signal showed a high concentration in the nuclear areas of the epithelial cells and a lower concentration over areas representing the basal lamina of renal tubules. A more diffuse distribution of cholesterol was also found over areas representing the cytoplasm or plasma membrane of the epithelial cells.
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