The major androgen‐dependent protease in dog prostate belongs to the kallikrein family: confirmation by partial amino acid sequencing
1984; Wiley; Volume: 175; Issue: 1 Linguagem: Inglês
10.1016/0014-5793(84)80557-8
ISSN1873-3468
AutoresClaude Lazure, Richard Leduc, Nabil G. Seidah, Michel Chrètien, Jean Dubé, Pierre Chapdelaine, Gilles Frenette, Robert Paquin, Roland R. Tremblay,
Tópico(s)Muscle metabolism and nutrition
ResumoCanine prostate fluids and seminal plasma contain a major androgen‐dependent protein which was identified as a proteolytic enzyme exhibiting an Arg‐esterase activity. This protease, as characterized, is shown to be present as a two‐chain structure held together by at least one disulfide bridge and composed of approximately 220 amino acids. Amino acid sequence determination of both chains has revealed a clear homology to other known amino acid sequences of serine proteases. Furthermore, the comparison of the presented 58 amino acids of the Arg‐esterase with the other sequences revealed a very strong homology (larger than 50%) to members of the kallikrein family. The two chain structure could thus result from autolysis of a single chain enzyme in the ‘kallikrein autolysis loop’. Amino acid composition of the canine prostatic enzyme suggests that it is related, but not identical, to pancreatic canine kallikrein.
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