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Local Infusion of the Nitric oxide Donor Sin-1 After Angioplasty . Effects on intimal hyperplasia in porcine coronary arteries

2003; SAGE Publishing; Volume: 44; Issue: 4 Linguagem: Inglês

10.1034/j.1600-0455.2003.00079.x

1600-0455

Jan Harnek, E. Zoucas, Rolf Sjuve, Peter Arner, Eva Ekblad, H. I. SCHOU, Valéria Perez‐de‐Sá, Unne Stenram,

Acute Myocardial Infarction Research

Purpose: To investigate the development of intimal hyperplasia in response to percutaneous transluminal coronary angioplasty (PTCA) followed by local delivery of the nitric oxide (NO) donor 3-morpholino-sydnonimine (SIN-1).Material and Methods: Overdilation PTCA was performed in coronary arteries in 20 healthy pigs.One of the dilated segments was additionally treated with local delivery of SIN-1 for 10 min.Segments distal to the treated part of the arteries served as controls.Arteries were radiographically depicted and analyzed after 1 and 8 weeks for actin, myosin and intermediate filaments (IF), nitric oxide synthetase (NOS) and histological evaluation.Results: Segments treated with PTCAþSIN-1 showed a significantly (p ¼ 0.03) larger luminal diameter compared with PTCA only treated segments.The luminal loss after SIN-1 was not significant compared with the diameter prior to treatment.Endothelial NOS content was significantly lower in the PTCAþSIN-1 group compared with the PTCA group after 1 (p ¼ 0.03) and 8 weeks (p ¼ 0.013).IF/actin ratio after 1 week was significantly increased in PTCA-treated segments compared with untreated controls (p ¼ 0.004), and compared with PTCAþSIN-1-treated segments (p ¼ 0.004).Conclusion: PTCA-induced intimal hyperplasia was potently inhibited by local delivery of the NO donor SIN-1.Momentary events at the time of injury play a significant role in the development of intimal hyperplasia and long-lasting downregulation of the endothelial NOS expression after SIN-1 exposure is suggested.The IF/actin ratio can be useful as an early marker of intimal hyperplasia.