Preparative, affinity chromatography of sheep gastric-mucins having blood-group Ii activity, and release of antigenically active oligosaccharides by alkaline-borohydride degradation
1981; Elsevier BV; Volume: 90; Issue: 2 Linguagem: Inglês
10.1016/s0008-6215(00)85926-1
ISSN1873-426X
AutoresEdwin Wood, Elizabeth F. Hounsell, Ten Feizi,
Tópico(s)Microbial Metabolites in Food Biotechnology
ResumoAbstract A large number of oligosaccharide fractions having blood-group Ii activity were obtained by degradation with alkaline borohydride of sheep gastric-glycoproteins which had been enriched for these blood-group activities by affinity chromatography on an anti-I adsorbent column. The approximate molecular weights of the oligosaccharides were estimated from their elution profiles on Bio-Gel P4 and from monosaccharide compositions. The fractions of smallest molecular weight with both I and i activities were mixtures of hexa- to octa-saccharides. From a comparison of the inhibitory activities of these with fractions of higher molecular weight and with synthetic oligosaccharides, it was concluded that the antigenic determinant recognised by anti-I Ma is a trisaccharide, whereas those recognised by other types of anti-I and by anti-i antibodies are longer than trisaccharides.
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