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Effect of Postharvest Storage on the Expression of the Apple Allergen Mal d 1

2006; American Chemical Society; Volume: 54; Issue: 16 Linguagem: Inglês

10.1021/jf060880m

1520-5118

Ana I. Sancho, Robert J. Foxall, Tom Browne, Rickmer Dey, Laurian Zuidmeer, Gorji Marzban, Keith W. Waldron, Ronald van Ree, Karin Hoffmann‐Sommergruber, Margit Laimer, E. N. Clare Mills,

Contact Dermatitis and Allergies

Consumption of fresh apples can cause allergy in susceptible individuals. A competitive enzyme-linked immunosorbent assay (ELISA) has been developed to determine Mal d 1 levels in apple pulp using a monoclonal antibody (BIP-1). The ELISA was able to rank ten cultivars according to their Mal d 1 content (between 3.8 and 72.5 μg/g pulp). For the first time, it has been demonstrated that growing conditions and postharvest storage, using three different treatments over a 5 month period in 2 consecutive years, increase Mal d 1 expression at a translational and transcriptional level (3.5- and 8.5-fold under controlled atmosphere storage). Expression of three major Mal d 1 isoforms was observed by real-time polymerase chain reaction over the 5 month storage period, and Mal d 1.02 was the most highly expressed isoform. In conclusion, Mal d 1 gene expression was significantly increased during modified atmosphere storage. Individuals suffering from birch pollen−apple allergy syndrome might experience fewer problems consuming freshly picked apples. Keywords: Apple; allergens; Bet v 1; cultivar; Mal d 1; Mal d 1 expression; PR 10; storage