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Kinetic study of biosurfactant production by Bacillus subtilis LAMI005 grown in clarified cashew apple juice

2012; Elsevier BV; Volume: 101; Linguagem: Inglês

10.1016/j.colsurfb.2012.06.011

1873-4367

Darlane Wellen Freitas de Oliveira, Ítalo Waldimiro Lima de França, Anne Kamilly Nogueira Félix, João Jeferson Lima Martins, Maria Estela Aparecida Giro, Vânia Maria Maciel Melo, Luciana Rocha Barros Gonçalves,

Toxic Organic Pollutants Impact

In this work a low cost medium for the production of a biosurfactant by Bacillus subtilis LAMI005 and the kinetics of surfactin production considering the effect of initial substrate concentration were investigated. First, cashew apple juice supplementation for optimal production of biosurfactant by B. subtilis LAMI005 was studied. The medium formulated with clarified cashew apple juice and distilled water, supplemented with 1.0 g/L of (NH4)2SO4, proved to be the best among the nutrients evaluated. The crude biosurfactant had the ability to decrease the surface tension of water to 30 dyne/cm, with a critical micelle concentration (CMC) of 63.0 mg/L. Emulsification experiments indicated that this biosurfactant effectively emulsified kerosene (IE24 = 67%) and soybean oil (IE24 = 64%). Furthermore, the emulsion stability was always very high. It was shown by biochemical analysis, IR spectra, that there is no qualitative differences in the composition of the crude biosurfactant from a standard sample of surfactin from B. subtilis.