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Modelling and analysis of the elution stage of “perfusion chromatography” effects of intraparticle convective velocity and microsphere size on system performance

1996; Elsevier BV; Volume: 724; Issue: 1-2 Linguagem: Inglês

10.1016/0021-9673(95)00952-3

1873-3778

Yihui Xu, A.I. Liapis,

Protein purification and stability

A mathematical model describing the dynamic elution (elution stage) in columns with spherical bidisperse perfusive or spherical bidisperse purely diffusive adsorbent particles is presented and its solution is obtained numerically. The desorption of bovine serum albumin (BSA) from spherical anion-exchange porous particles was studied for different values of the intraparticle Peclet numbers of the adsorbate, (Peintra)a, and eluent, (Peintra)e, and of the microsphere diameter, dm. It was found that as (Peintra)a increases, the asymmetry of the elution peak of the adsorbate (at the exit of the column) decreases, the peak height increases and the time, Te, required for the elution of the adsorbate decreases. The results also show that Te decreases, and the average bulk concentration of the adsorbate, Cd, bulk, in the solution collected at the exit of the column and the concentration factor, CF, increase as the diameter of the microsphere, dm, decrease and the value of (Peintra)a increase for the adsorbent particles used in the column. Furthermore, the results clearly show that for given values of dm and (Peintra)a, the systems with reverse flow during the elution stage exhibit better performance than that obtained from the system where the eluent flows in the same direction in the column during the elution stage as was the direction of flow of the adsorbate in the column during the adsorption stage.