Nucleotide sequence of the complementary DNA for human Pit-1/GHF-1

1992; Elsevier BV; Volume: 1129; Issue: 2 Linguagem: Inglês

10.1016/0167-4781(92)90494-k

ISSN

1879-2634

Autores

Ke-ita Tatsumi, Tsugunori Notomi, Nobuyuki Amino, K Miyai,

Tópico(s)

Pituitary Gland Disorders and Treatments

Resumo

Human cDNA clones encoding Pit-1/GHF-1, a pituitary-specific DNA binding factor, were obtained by PCR following reverse transcription of human pituitary RNA. It is approx. 1.3 kb in size with 0.1 kb 5′ non-coding region, 0.9 kb protein-coding region and 0.3 kb 3′ non-coding region. The predicted human Pit-1/GHF-1 peptide structure has 291 amino acids and is highly conserved among mouse, rat and bovine. In addition, the 5′ non-coding region is highly conserved with rat pit-1/GHF-1 sequence to the transcription start site.

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