Determination of the enantiomers of bunolol in human urine by high-performance liquid chromatography on a chiral AGP stationary phase and identification of their metabolites by gas chromatography—mass spectrometry
1994; Elsevier BV; Volume: 660; Issue: 2 Linguagem: Inglês
10.1016/0378-4347(94)00295-9
ISSN1872-812X
AutoresF Li, Sam F. Cooper, Michel Côté, Christiane Ayotte,
Tópico(s)Analytical Methods in Pharmaceuticals
ResumoA high-performance liquid chromatographic method using a chiral AGP column was developed to screen and determine the enantiomers of bunolol in human urine. The recovery of (+)- and (-)-bunolol from urine was 91.79-95.23% at different concentrations. The coefficients of variation (C.V.) were less than 2.1 and 2.3% for intra- and inter-assays, respectively. Urinary metabolites were detected using GC-MS after derivatization with N-methyl(trimethylsilyl)trifluroacetamide. The influences of pH and modifier on a chiral AGP column were studied.
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