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Multimodal nonlinear spectral microscopy based on a femtosecond Cr:forsterite laser

2001; Optica Publishing Group; Volume: 26; Issue: 23 Linguagem: Inglês

10.1364/ol.26.001909

1539-4794

Shi‐Wei Chu, I-Hsiu Chen, Tzu‐Ming Liu, Ping Chin Chen, Chi‐Kuang Sun, Bai-Ling Lin,

Spectroscopy Techniques in Biomedical and Chemical Research

We demonstrate a novel multimodal nonlinear spectral microscopy based on a femtosecond Cr:forsterite laser at 1230 nm. By acquiring the whole nonlinear spectrum in the visible and near-NIR region, this novel technique allows a combination of different imaging modalities, including second-harmonic generation, third-harmonic generation, and multiple-photon fluorescence. Combined with the selected excitation wavelength, which is located in the IR transparency window, this microscopic technique can provide high penetration depth with reduced damage and is ideal for studying living cells.