Portal de Periódicos da CAPES

Update on Primary HIV-1 Resistance in Argentina

2006; Lippincott Williams & Wilkins; Volume: 42; Issue: 4 Linguagem: Inglês

10.1097/01.qai.0000222285.44460.e2

1944-7884

Alejandro Petroni, Gabriel Deluchi, Daniel Pryluka, Flavio Rotryng, Raúl Bortolozzi, Gustavo Lopardo, María Belén Bouzas, Inés Zapiola, Daniela Garone, Claudia Rodríguez, Eduardo Chiocconi, María Esther Lázaro, Fernando Murano, Aldo Maranzana, Stella Maris Oliva, M. Desamparados Martínez Aparicio, Marcelo A. Beltrán, Jorge Benetucci,

HIV/AIDS Research and Interventions

Here we present a survey including 52 drug-naive recently HIV-1-infected subjects from Buenos Aires City and province (79%) and 3 other regions in Argentina (21%). Recent infections were established from previous negative serology (32/52), indeterminate Western blot (12/52), or acute retroviral syndrome after high-risk HIV exposure (8/52) within 9 months before genotyping (median time, 4.2 months). Genotyping was performed from plasma by sequencing both protease and reverse transcriptase. Phylogenetic analysis combined with bootscanning resulted in 21 subtype B sequences and 31 B/F recombinants (RecBF). On protease, minor resistance-related mutations were found in both subtype B and RecBF with low frequencies. The substitution L89M, recently suggested as a resistance-related mutation in some subtype F viruses, was observed in 1 RecBF. On reverse transcriptase, major resistance-related mutations were found in 4 of 52 (7.7%) patients from different health centers: M41L (subtype B) and K103N+/-P225H (1 RecBF and 2 subtype B). The greater than 5% resistance threshold found indicates a need for sentinel resistance surveillances calling for an update in the current resistance testing guidelines in Argentina.