Dehydroluciferyl‐AMP is the main intermediate in the luciferin dependent synthesis of Ap 4 A catalyzed by firefly luciferase
1998; Wiley; Volume: 438; Issue: 3 Linguagem: Inglês
10.1016/s0014-5793(98)01301-5
ISSN1873-3468
AutoresRui Fontes, Begoña Ortiz, Anabel de Diego, Antonio Sillero, Marı́a A. Günther Sillero,
Tópico(s)Retinal Development and Disorders
ResumoIt was previously assumed that E·LH 2 ‐AMP was the intermediate complex in the synthesis of Ap 4 A catalyzed by firefly luciferase (EC 1.13.12.7), when luciferin (LH 2 ) was used as cofactor. However, here we show that LH 2 is partly transformed, shortly after the onset of the luciferase reaction, to dehydroluciferin (L) with formation of an E·L‐AMP complex which is the main intermediate for the synthesis of Ap 4 A. Formation of three more derivatives of LH 2 were also observed, related to the production of light by the enzyme. CoA, a known stimulator of light production, inhibits the synthesis of Ap 4 A by reacting with the E·L‐AMP complex and yielding L‐CoA.
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