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Synthesis and Screening of a Molecularly Imprinted Polymer Library Targeted for Penicillin G

2002; American Chemical Society; Volume: 5; Issue: 1 Linguagem: Inglês

10.1021/cc020051n

1520-4774

Josefine Cederfur, Yuxin Pei, Zihui Meng, Maria Kempe,

Antibiotics Pharmacokinetics and Efficacy

A library of molecularly imprinted polymers (MIPs) was synthesized by radical bulk polymerization using the β-lactam antibiotic penicillin G as the template. Diversity of the library was obtained by combining various functionalized monomers and cross-linkers and by varying the stoichiometry and the concentration of the components in the prepolymerization mixtures. The library was screened for selectivity to penicillin G by a radioligand binding assay and was compared to a corresponding control library. The best MIP candidate, showing the highest selectivity for penicillin G, was prepared from methacrylic acid and trimethylolpropane trimethacrylate as the functionalized monomer and cross-linker, respectively. Cross-reactivity studies with other β-lactam antibiotics showed a low cross-reactivity of penicillin V (15%), ampicillin (16%), and amoxicillin (19%). Nafcillin and oxacillin showed less cross-reactivity (<1%). Cross-reaction with a cephalosporin antibiotic (cephapirin) and structurally nonrelated antibiotics (chloramphenicol, tetracycline, dapsone, and erythromycin) was less than 0.01%.