The Kinase-null EphB6 Receptor Undergoes Transphosphorylation in a Complex with EphB1
2002; Elsevier BV; Volume: 277; Issue: 6 Linguagem: Inglês
10.1074/jbc.m108011200
ISSN1083-351X
AutoresAndrew Freywald, Nigel Sharfe, Chaim M. Roifman,
Tópico(s)Hippo pathway signaling and YAP/TAZ
ResumoUniquely for the Eph family of receptor tyrosine kinases, the EphB6 receptor is catalytically inactive due to the alteration of several critical residues in its kinase domain. This has cast doubt upon its ability to participate in cytoplasmic signaling events. We show here that despite its lack of kinase activity, EphB6 undergoes inducible tyrosine phosphorylation upon stimulation with the Eph-B receptor subfamily ligand ephrin-B1. We also demonstrate, for the first time, evidence of cross-talk between Eph receptors. Overexpression of a catalytically active member of the Eph-B subfamily, EphB1, resulted in increased EphB6 phosphorylation. EphB1-induced EphB6 phosphorylation was ligand-dependent and required the functional catalytic activity of EphB1. EphB1 not only transphosphorylated EphB6, but together they also formed a stable hetero-complex. In addition, we identify the proto-oncogene c-Cbl as an EphB6-binding protein. Although EphB6-Cbl association appeared to be constitutive, Cbl required a functional phosphotyrosine binding domain in order to bind the receptor, whereas its RING finger motif ubiquitin-transfer domain was not necessary. Our findings demonstrate that EphB6 is an actively signaling receptor that undergoes transphosphorylation upon ligand binding and that can initiate specific cytoplasmic signaling events. Uniquely for the Eph family of receptor tyrosine kinases, the EphB6 receptor is catalytically inactive due to the alteration of several critical residues in its kinase domain. This has cast doubt upon its ability to participate in cytoplasmic signaling events. We show here that despite its lack of kinase activity, EphB6 undergoes inducible tyrosine phosphorylation upon stimulation with the Eph-B receptor subfamily ligand ephrin-B1. We also demonstrate, for the first time, evidence of cross-talk between Eph receptors. Overexpression of a catalytically active member of the Eph-B subfamily, EphB1, resulted in increased EphB6 phosphorylation. EphB1-induced EphB6 phosphorylation was ligand-dependent and required the functional catalytic activity of EphB1. EphB1 not only transphosphorylated EphB6, but together they also formed a stable hetero-complex. In addition, we identify the proto-oncogene c-Cbl as an EphB6-binding protein. Although EphB6-Cbl association appeared to be constitutive, Cbl required a functional phosphotyrosine binding domain in order to bind the receptor, whereas its RING finger motif ubiquitin-transfer domain was not necessary. 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EphB6 forms a hetero-receptor complex with co-expressed EphB1 receptor and undergoes transphosphorylation by the catalytically active EphB1 in a ligand-dependent manner. We also demonstrate that the EphB6 receptor associates with the c-Cbl oncogene. Cbl participates in the signaling pathways of many receptors, functioning as a regulator of receptor activity and binding a variety of signal-transducing molecules (41Fournel M. Davidson D. Weil R. Veillette A. J. Exp. Med. 1996; 183: 301-306Crossref PubMed Scopus (123) Google Scholar, 42Lupher Jr., M.L. Reedquist K.A. Miyake S. Langdon W.Y. Band H. J. Biol. Chem. 1996; 271: 24063-24068Abstract Full Text Full Text PDF PubMed Scopus (165) Google Scholar, 43Lupher Jr., M.L. Songyang Z. Shoelson S.E. Cantley L.C. Band H. J. Biol. Chem. 1997; 272: 33140-33144Abstract Full Text Full Text PDF PubMed Scopus (184) Google Scholar, 44Lupher Jr., M.L. Rao N. Lill N.L. Andoniou C.E. Miyake S. Clark E.A. Druker B. Band H. J. Biol. 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Cbl binding to EphB6 was found to be constitutive, and oncogenic 70-Z Cbl, containing a deleted RING finger motif, bound EphB6 essentially like wild type Cbl. In contrast, binding was lost upon the introduction of a Cbl G306E "loss of function" mutation. This mutation ablates the function of the Cbl PTB domain, a domain that is also crucial for interaction with other receptor tyrosine kinases (55Bonita D.P. Miyake S. Lupher Jr., M.L. Langdon W.Y. Band H. Mol. Cell. Biol. 1997; 17: 4597-4610Crossref PubMed Google Scholar). Monoclonal anti-phosphotyrosine was obtained from Upstate Biotechnology, Inc. (Lake Placid, NY). Antibodies to EphB6, Myc, Cbl, and EphB1 were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Soluble dimerized ephrin-B1 and soluble EphB6 receptors were purchased from R&D Systems (Minneapolis, MN). Anti-T7 was purchased from Novagen. cDNAs for Cbl, EphB1, EphB6, ephrin-A1, and ephrin-B1 were cloned from normal human thymocyte RNA by reverse transcriptase PCR into the expression vector pcDNA3 (Invitrogen) and sequenced. Mutants of Cbl (G306E and deletion homologous to murine 70Z) and EphB1 (truncation of 102 C-terminal amino acids) were created using PCR to introduce the required base changes using cloned cDNAs as the template. The following sense oligomers and perfect match antisense oligomers were utilized for site-directed mutagenesis. Cbl G306E, 5′-cagtgggctattgagtatgttactgct; 70-Z Cbl, 5′-gaccatatcaaagtgacccagatatgtgctgaaaatgac. The deletion of the B1 receptor was created with the following antisense oligomer: 5′-gatcatcttatctagggtgttgac. Kinase-null B1 was created by mutating lysine 651 to glutamine (K651Q). The resulting cDNAs were cloned and sequenced to confirm the mutations. Myc-tagged versions of EphB6 and of the truncated EphB1 receptor were generated by insertion of a Myc tag, and all constructs were verified by sequencing. Expression of wild type proteins and mutants was examined by transfection in COS-7 cells and Western blotting with appropriate antibodies. By in vitro kinase assay, both the wild type and truncated forms of EphB1 were active kinases (data not shown). COS-7, HEK 293, and NIH 3T3 cells were routinely transiently transfected using the lipid reagent LipofectAMINE (Invitrogen). The DNA/lipid mixtures were applied to the cells for 5 h in the absence of serum before the addition of complete medium. Cells were given 72 h to express the transfected proteins before harvest. To assay for stimulation with membrane-bound forms of the ephrin ligands, receptor-expressing cells were resuspended using 2.5 mm EDTA, and after washing, they were overlaid on a confluent monolayer of control or ligand-expressing cells. After incubation at 37 °C for 1 h, all the cells were solubilized in 1% Triton lysis buffer. Soluble ephrin-B1-Fc fusion protein dimer was purchased from R&D Systems. The dimeric ephrin-B1 fusion protein was precomplexed with F(ab′)2 goat anti-human Fc (Pierce) to form oligomers. F(ab′)2 goat anti-human Fc was used as a control (no stimulation) where necessary. Although murine ephrin-B1 was utilized, this effectively induced human EphB6 phosphorylation. Cells were quickly resuspended in ice-cold lysis buffer consisting of 50 mmTris-HCl, pH 7.5, 150 mm NaCl, 10% glycerol, 1% Triton X-100, 1 mm ethylene glycol-bis (β-aminoethylether)-N, EGTA, 10 μg/ml leupeptin, 10 μg/ml aprotinin, 1 mm phenylmethylsulfonyl fluoride, 1 mm sodium orthovanadate, and 50 mm NaF. After solubilization on ice for 15 min, debris was removed by centrifugation at 12,000 × g for 10 min at 4 °C. Antibodies and 20 μl of 50% protein G-Sepharose were added to cleared lysates and incubated at 4 °C with constant shaking for 12–16 h. Immunoprecipitates were collected by a brief centrifugation and washed three to four times in lysis buffer (without phenylmethylsulfonyl fluoride) before the addition of SDS sample buffer. Samples were separated on SDS-polyacrylamide gels and transferred to nitrocellulose membranes (Amersham Biosciences, Inc.). Membranes were blocked overnight at 4 °C with 7% blotting grade non-fat milk (Bio-Rad, Richmond, CA) in PBS (phosphate-buffered saline). Immunoblotting antibodies were added at optimal dilutions in PBS-T (PBS with 0.1% Tween 20) and incubated at 4 °C overnight. After extensive washing with PBS-T, bound antibodies were detected using horseradish peroxidase-conjugated donkey anti-rabbit or sheep anti-mouse antibodies and ECL reagents (Amersham Biosciences, Inc.). EphB receptors are activated by membrane-bound ephrin-B ligands, demonstrating highly degenerate specificity with ephrin-B1 and ephrin-B2 activating most EphB receptors. To determine whether the catalytically inactive EphB6 receptor could be tyrosine-phosphorylated in response to ephrin-B ligand stimulation, we transiently expressed Myc-tagged human EphB6 in COS-7 cells and exposed the cells to ephrin-B1. To provide cell surface-expressed ligands, we transfected COS-7 cells with pcDNA3 expression vector containing either ephrin-A1 or ephrin-B1 cDNA. Ligand expression was verified by immunoblotting (Fig. 1A,bottom panel). Western blot analysis did not detect endogenous ephrin-B1 expression in COS-7 cells or several other adherent cell lines (NIH 3T3; HEK 293; −, not shown). EphB6-expressing cells were overlaid on cells transfected with ephrin-B1, ephrin-A1, or empty vector and co-incubated for 1 h at 37 °C. The EphB6 receptor was then precipitated with anti-Myc and immunoblotted with anti-phosphotyrosine antibody. Stimulation of EphB6 with ephrin-B1-expressing cells resulted in a marked increase in EphB6 tyrosine phosphorylation, whereas co-incubation with ephrin-A1-expressing or control cells had no effect (Fig.1A, top panel). The increase in EphB6 tyrosine phosphorylation upon co-incubation with ephrin-B1-expressing cells was also observed in EphB6-transfected NIH 3T3 fibroblasts and HEK 293 human embryonic kidney cells, demonstrating that the effect was not cell-specific (Fig. 1B). EphB6 tyrosine phosphorylation was detectable within 20 min of simulation and increased thereafter (Fig.2A). The time required to observe EphB6 phosphorylation was relatively long, presumably due to the delay in the initiation of cell-cell contact until the overlaid cells settled onto the ligand-expressing monolayer. EphB6 phosphorylation was dependent upon ligand concentration as transfection of increasing amounts of ephrin-B1 cDNA elicited greater EphB6 phosphorylation responses. (Fig. 2B). In contrast to soluble monomers of ephrins, which inhibit Eph receptor signaling, dimerized or oligomerized forms can stimulate receptor autophosphorylation and signaling (19Davis S. Gale N.W. Aldrich T.H. Maisonpierre P.C. Lhotak V. Pawson T. Goldfarb M. Yancopoulos G.D. Science. 1994; 266: 816-819Crossref PubMed Scopus (627) Google Scholar, 20Sakano S. Serizawa R. Inada T. Iwama A. Itoh A. Kato C. Shimizu Y. Shinkai F. Shimizu R. Kondo S. Ohno M. Suda T. Oncogene. 1996; 13: 813-822PubMed Google Scholar). Soluble dimerized ephrin-B1 was also found to induce EphB6 phosphorylation (Fig.3). Although dimerized soluble murine ephrin-B1 was utilized for this experiment, this induced EphB6 phosphorylation as effectively as the membrane-expressed human ephrin-B used in Fig. 1. The induction of EphB6 phosphorylation by soluble dimerized ephrin-B1 could be completely blocked by the addition to the medium of a soluble form of the EphB6 receptor extracellular domain (5 μg/ml) (Fig. 3), strongly suggesting the existence of a direct interaction between ephrin-B1 and EphB6 receptors. Because EphB6 is catalytically inactive, the tyrosine phosphorylation observed upon ephrin-B1 stimulation must reflect the activity of an endogenous tyrosine kinase. To pose an analogy with the catalytically inactive ErbB-3 receptor of the EGFR family, EphB6 may be transphosphorylated by catalytically active members of the Eph family. To investigate this possibility, we co-expressed EphB6 with the human EphB1 receptor in COS-7 cells. COS-7 cells demonstrated a very low level of endogenous EphB1 (not shown). However, when overexpressed, EphB1 was found to become constitutively activated, as observed previously with the EphB3 receptor (58Bohme B. VandenBos T. Cerretti D.P. Park L.S. Holtrich U. Rubsamen W.H. Strebhardt K. J. Biol. Chem. 1996; 271: 24747-24752Abstract Full Text Full Text PDF PubMed Scopus (64) Google Scholar, 59Hock B. Bohme B. Karn T. Feller S. Rubsamen-Waigmann H. Strebhardt K. Oncogene. 1998; 17: 255-260Crossref PubMed Scopus (55) Google Scholar). When co-expressed with EphB1, EphB6 did indeed appear to undergo significant tyrosine phosphorylation, transphosphorylating in a manner analogous to the EGF family receptor ErbB-3 (Fig.4A). Only catalytically active EphB1 induced EphB6 phosphorylation as no effect was observed upon EphB6 co-expression with a kinase-null K651Q EphB1 mutant (Fig.4B, B1-KD). In NIH 3T3 fibroblasts, where the basal activity of EphB1 was determined to be lower than in 293 or COS-7 cells, EphB6 transphosphorylation by EphB1 occurred in a ligand-dependent manner (Fig. 4C). The ability of EphB1 to induce EphB6 phosphorylation suggested that the two receptors might be complexed when co-expressed. Indeed, EphB6 and EphB1 could be co-immunoprecipitated from co-transfected COS-7 cells (Fig. 4D), suggesting the existence of a direct interaction between the receptors and the formation of a relatively stable complex. Furthermore, this suggested that the catalytically active EphB1 receptor might directly phosphorylate EphB6. The co-precipitation of EphB1 and EphB6, combined with their similar electrophoretic mobility, raised the possibility that the apparent phosphorylation of EphB6 observed upon EphB1 co-expression could be due to co-precipitation of phosphorylated EphB1. To unambiguously distinguish between the receptors, we constructed a Myc-tagged truncated EphB1 receptor lacking 102 C-terminal residues but with its kinase domain intact (B1-Tr). The truncated receptor was constitutively tyrosine-phosphorylated, similar to the wild type EphB1 but now clearly smaller than EphB6. Similar to overexpression of the wild type EphB1 receptor, overexpression of the truncated EphB1 also increased EphB6 phosphorylation in a ligand-independent manner (Fig.5, A and B). In sum, these findings demonstrated that EphB6 could form hetero-receptor complexes with an active Eph receptor and undergo transphosphorylation by the associated receptor. They also suggest that ephrin-B1-induced EphB6 phosphorylation in the absence of co-transfected EphB1 may similarly result from transphosphorylation by endogenous active Eph receptors. Cbl participates in the signaling pathways of many receptors, functioning as a regulator of receptor activity through the initiation of receptor ubiquitination and inducibly binding a variety of signal-transducing molecules (41Fournel M. Davidson D. Weil R. Veillette A. J. Exp. Med. 1996; 183: 301-306Crossref PubMed Scopus (123) Google Scholar, 42Lupher Jr., M.L. Reedquist K.A. Miyake S. Langdon W.Y. Band H. J. Biol. 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Murphy M.A. Bowtell D.D. Stanley E.R. EMBO J. 1999; 18: 3616-3628Crossref PubMed Scopus (250) Google Scholar, 50Levkowitz G. Waterman H. Zamir E. Kam Z. Oved S. Langdon W.Y. Beguinot L. Geiger B. Yarden Y. Genes Dev. 1998; 12: 3663-3674Crossref PubMed Scopus (713) Google Scholar, 51Miyake S. Lupher Jr., M.L. Druker B. Band H. Proc. Natl. Acad. Sci. U. S. A. 1998; 95: 7927-7932Crossref PubMed Scopus (234) Google Scholar). The ability of Cbl to interact with a variety of tyrosine kinase receptors led us to examine whether Eph receptors might also bind Cbl. We co-expressed the EphB6 and EphB1 receptors in COS-7 cells with human c-Cbl and examined their potential to associate. Although we did not detect any association between Cbl and the catalytically active EphB1 receptor, Cbl strongly co-precipitated with EphB6 (Fig.6A). This association appeared to be constitutive because stimulation with ephrin-B1-expressing cells altered neither the level of EphB6-Cbl association nor the tyrosine phosphorylation of Cbl (not shown). Although COS-7 cells express endogenous Cbl, association with EphB6 could only be consistently detected upon overexpression of both Cbl and the receptor, similarly to other receptor tyrosine kinases. To further characterize the interaction, we examined the binding of two well characterized mutants of Cbl to the EphB6 receptor. The first, G306E (Cbl*), was initially identified as a mutation in the Caenorhabditis elegans Cbl orthologue sli-1 (60Jongeward G.D. Clandinin T.R. Sternberg P.W. Genetics. 1995; 139: 1553-1566Crossref PubMed Google Scholar), which causes loss of Cbl binding to the ErbB1 and PDGF receptors (55Bonita D.P. Miyake S. Lupher Jr., M.L. Langdon W.Y. Band H. Mol. Cell. Biol. 1997; 17: 4597-4610Crossref PubMed Google Scholar, 61Thien C.B. Langdon W.Y. Oncogene. 1997; 14: 2239-2249Crossref PubMed Scopus (66) Google Scholar). The G306E mutation disrupts the function of the PTB domain of Cbl, through which it binds phosphorylated tyrosine residues. The second naturally occurring mutant, 70-Z Cbl (Cbl**), was first isolated as an oncogene from a murine B cell line (62Blake T.J. Shapiro M. Morse H.C.d. Langdon W.Y. Oncogene. 1991; 6: 653-657PubMed Google Scholar). 70-Z Cbl contains an internal 17-amino acid deletion in the RING finger domain of Cbl. In co-immunoprecipitation assays, the 70-Z mutation had little effect upon Cbl binding to the EphB6 receptor, whereas the G306E point mutation completely abolished association (Fig. 6B). Thus, although Cbl-EphB6 association appeared to be constitutive, it would seem to be dependent upon the Cbl PTB domain and therefore probably dependent upon tyrosine phosphorylation of either the EphB6 receptor or an adapter molecule. Although the general structure of EphB6 is typical of the EphB receptors, its kinase domain contains numerous alterations to critical catalytic residues, and neither murine nor human EphB6 exhibits kinase activity (38Gurniak C.B. Berg L.J. Oncogene. 1996; 13: 777-786PubMed Google Scholar, 39Matsuoka H. Iwata N. Ito M. Shimoyama M. Nagata A. Chihara K. Takai S. Matsui T. Biochem. Biophys. Res. Commun. 1997; 235: 487-492Crossref PubMed Scopus (50) Google Scholar). We have demonstrated that despite these structural abnormalities, the human EphB6 receptor responds to ephrin-B1 stimulation by undergoing tyrosine phosphorylation. In addition, we show that this EphB6 tyrosine phosphorylation can be provided by a catalytically active Eph receptor, in particular by EphB1. While EphB1 receptor transphosphorylation of EphB6 was observed in co-transfected cells, EphB6 may potentially interact with multiple members of the EphB subfamily in vivo. The ability to co-immunoprecipitate EphB1 and EphB6 suggested that transphosphorylation occurred as the result of the formation of an Eph receptor hetero-oligomeric complex on the plasma membrane. Indeed, lacking catalytic activity, EphB6 is unlikely to operate as an independent receptor but rather as part of such a hetero-oligomeric signaling complex with active EphB receptors in vivo. Until now, ErbB-3 of the EGF receptor family has been the only example of a transphosphorylated kinase-inactive receptor (40Pinkas K.R. Soussan L. Waterman H. Levkowitz G. Alroy I. Klapper L. Lavi S. Seger R. Ratzkin B.J. Sela M. Yarden Y. EMBO J. 1996; 15: 2452-2467Crossref PubMed Scopus (691) Google Scholar); however, our findings suggest that this may be a common mechanism for signaling through catalytically inactive receptor tyrosine kinases. The ErbB-3 receptor acts to modulate the intensity and duration of signaling by its active partner (40Pinkas K.R. Soussan L. Waterman H. Levkowitz G. Alroy I. Klapper L. Lavi S. Seger R. Ratzkin B.J. Sela M. Yarden Y. EMBO J. 1996; 15: 2452-2467Crossref PubMed Scopus (691) Google Scholar, 50Levkowitz G. Waterman H. Zamir E. Kam Z. Oved S. Langdon W.Y. Beguinot L. Geiger B. Yarden Y. Genes Dev. 1998; 12: 3663-3674Crossref PubMed Scopus (713) Google Scholar), and transphosphorylation results in the recruitment of Shc and phosphatidylinositol 3-kinase specifically to the ErbB-3 receptor chain (63Kim H.H. Sierke S.L. Koland J.G. J. Biol. Chem. 1994; 269: 24747-24755Abstract Full Text PDF PubMed Google Scholar, 64Waterman H. Alroy I. Strano S. Seger R. Yarden Y. EMBO J. 1999; 18: 3348-3358Crossref PubMed Scopus (83) Google Scholar). In a similar fashion, the catalytically inactive EphB6 receptor may recruit specific cytoplasmic signaling molecules and thus modulate cell responses induced by ephrin-B1 stimulation. In addition to initiating specific signaling pathways upon transphosphorylation, the catalytically inactive ErbB3 receptor is known to regulate the behavior of other EGFR family members. Similarly, as all EphB receptors are essentially activated by the same ligands, it is highly likely that they are all involved in functional cross-talk with EphB6, inducing EphB6 signaling through transphosphorylation and possibly undergoing modulation in return. EphB6, similar to the ErbB1 and PDGF receptors, was found to physically associate with Cbl. The G315E mutation of the C. elegans Cbl orthologue Sli-1 prevents interaction with the nematode ErbB protein (let-23) (60Jongeward G.D. Clandinin T.R. Sternberg P.W. Genetics. 1995; 139: 1553-1566Crossref PubMed Google Scholar), and the analogous Cbl mutation disrupts binding to PDGF and ErbB-1 receptors (55Bonita D.P. Miyake S. Lupher Jr., M.L. Langdon W.Y. Band H. Mol. Cell. Biol. 1997; 17: 4597-4610Crossref PubMed Google Scholar, 61Thien C.B. Langdon W.Y. Oncogene. 1997; 14: 2239-2249Crossref PubMed Scopus (66) Google Scholar). This mutation also abolished Cbl association with EphB6. The G306E mutation disrupts the Cbl PTB domain, suggesting that EphB6 phosphorylation may be important for Cbl binding, although we did not detect ligand-induced binding of Cbl to EphB6 despite increased receptor tyrosine phosphorylation. However, it was notable in our experiments that detectable basal levels of EphB6 phosphorylation were evident, suggesting that the Cbl binding site of EphB6 could be constitutively phosphorylated. Alternatively, Cbl binding may be indirect, possibly through a phosphorylated adapter protein. This could explain both Cbl binding to a catalytically inactive mutant of the EGFR (50Levkowitz G. Waterman H. Zamir E. Kam Z. Oved S. Langdon W.Y. Beguinot L. Geiger B. Yarden Y. Genes Dev. 1998; 12: 3663-3674Crossref PubMed Scopus (713) Google Scholar) and the constitutive association of Cbl and EphB6 observed in our experiments. Although EGF stimulation of the ErbB-1 receptor induces tyrosine phosphorylation of Cbl (56Levkowitz G. Klapper L.N. Tzahar E. Freywald A. Sela M. Yarden Y. Oncogene. 1996; 12: 1117-1125PubMed Google Scholar), we did not detect increased Cbl phosphorylation upon ephrin-B1 stimulation of EphB6 (not shown). This lack of Cbl phosphorylation presumably reflects the absence of EphB6 catalytic activity, suggesting that EphB6 may simply recruit Cbl to the cell membrane and into Eph receptor complexes rather than modifying its function through phosphorylation. The absence of inducible Cbl phosphorylation also suggests that it is not a substrate of the catalytically active EphB6 partner. Cbl is known to interact with regulators of cytoskeletal rearrangement, and recruitment of Cbl to sites of cell-cell contact by Eph receptors could therefore be important for the regulation of cell-cell interaction. The ability of Cbl to bind EphB6 also raises the possibility that EphB6 expression may be regulated by Cbl-mediated modification. It is now clear that Cbl is responsible for the physical down-regulation of many receptors through the induction of receptor ubiquitination (49Lee P.S. Wang Y. Dominguez M.G. Yeung Y.G. Murphy M.A. Bowtell D.D. Stanley E.R. EMBO J. 1999; 18: 3616-3628Crossref PubMed Scopus (250) Google Scholar, 50Levkowitz G. Waterman H. Zamir E. Kam Z. Oved S. Langdon W.Y. Beguinot L. Geiger B. Yarden Y. Genes Dev. 1998; 12: 3663-3674Crossref PubMed Scopus (713) Google Scholar, 51Miyake S. Lupher Jr., M.L. Druker B. Band H. Proc. Natl. Acad. Sci. U. S. A. 1998; 95: 7927-7932Crossref PubMed Scopus (234) Google Scholar). The addition of ubiquitin to the lysine residues of a protein targets it for degradation, either in cytoplasmic proteasomes or in the lysosomal compartment (57Hershko A. Ciechanover A. Annu. Rev. Biochem. 1998; 67: 425-479Crossref PubMed Scopus (6736) Google Scholar). Cbl binding induces ubiquitination of the EGF, PDGF, and CSF receptors (49Lee P.S. Wang Y. Dominguez M.G. Yeung Y.G. Murphy M.A. Bowtell D.D. Stanley E.R. EMBO J. 1999; 18: 3616-3628Crossref PubMed Scopus (250) Google Scholar, 50Levkowitz G. Waterman H. Zamir E. Kam Z. Oved S. Langdon W.Y. Beguinot L. Geiger B. Yarden Y. Genes Dev. 1998; 12: 3663-3674Crossref PubMed Scopus (713) Google Scholar, 52Miyake S. Mullane-Robinson K.P. Lill N.L. Douillard P. Band H. J. Biol. Chem. 1999; 274: 16619-16628Abstract Full Text Full Text PDF PubMed Scopus (154) Google Scholar, 53Wang Y. Yeung Y.G. Stanley E.R. J. Cell. Biochem. 1999; 72: 119-134Crossref PubMed Scopus (78) Google Scholar), an ability derived from its RING finger domain (54Waterman H. Levkowitz G. Alroy I. Yarden Y. J. Biol. Chem. 1999; 274: 22151-22154Abstract Full Text Full Text PDF PubMed Scopus (257) Google Scholar). As all receptors binding to Cbl undergo ubiquitination, it is likely that EphB6 is similarly regulated. In summary, we have demonstrated that despite its lack of kinase activity, EphB6 is an actively signaling receptor that undergoes ligand-inducible transphosphorylation and initiates specific cytoplasmic signaling events. Furthermore, we have also shown, for the first time, evidence of cross-talk between members of the Eph receptor family, demonstrating the innate complexity of the Eph receptor signaling system and the potential sophistication in responses to a single ephrin. We thank Ilan Braudi for technical assistance.
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