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How sigma docks to RNA polymerase and what sigma does

2001; Elsevier BV; Volume: 4; Issue: 2 Linguagem: Inglês

10.1016/s1369-5274(00)00177-6

1879-0364

Richard R. Burgess, Larry Anthony,

Bacteriophages and microbial interactions

It is clear that multiple sites of interaction exist between sigmas and core subunits, likely reflecting the changing pattern of interactions that occur sequentially during the complex process of holoenzyme formation, open promoter formation, and initiation of transcription. Recent studies have revealed that a major site of interaction of Escherichia coli sigma factors is the amino acid 260–309 coiled-coil region of the β′ subunit of core RNA polymerase. This region of β′ interacts with region 2.1–2.2 of σ70. Binding of this region of β′ to σ70 triggers a conformational change in sigma that allows it to bind to a −10 nontemplate promoter DNA strand oligonucleotide.